蝉拟青霉产透明质酸最佳液体培养基的筛选

Screen of Optimum Broth of Paecilomyces cicadae for Producing Hyaluronic Acid

本研究利用硫酸咔唑法检测透明质酸含量,考察了碳源、氮源和无机盐对蝉拟青霉发酵液中透明质酸含量的影响;实验结果表明,蝉拟青霉产透明质酸的最佳碳源、氮源和无机盐分别为:6%的乳糖、0.5%的牛肉膏与酵母膏的混合物(1:1)以及0.1%的MgSO4·7H2O。

The effects of carbon source, nitrogen source and mineral salt on hyaluronic acid （HA） concentration in Paecilomyces cicadae broth were determined by the method of sulfuric acid carbazole. The results showed that the optimum liquid medium formula of Paecilomyces cicadae for producing HA is lactose （6%）, mixture of beef extract （0.5%） and yeast extract（at the ratio of 1：1） and MgSO4 . 7H20 （0.1%）.