以淀粉废水、麦芽根制备生物材料——短梗霉多糖

Preparation of Biomaterial-pullulan with Starch Waste Water and Malt Root

短梗霉多糖是一种具有重要应用价值的微生物胞外多糖,淀粉废水是加工淀粉过程中不可避免的一种副产物,营养丰富;麦芽根为麦芽制造过程中的副产物,含有丰富的淀粉酶、麦芽酶、果酸酶及蛋白酶,富含B族维生素,并含有大量未知生长因子。因此,利用麦芽根所含有的丰富酶系和生长因子来酶解淀粉工业废水,便于出芽短梗霉菌的生长代谢。实验表明,具有一定的可行性。控制培养基初始pH为6.5、发酵温度为28～30℃、转速为180r/min、发酵时间120h的发酵工艺,较适合于出芽短梗霉菌利用麦芽根与淀粉废水的混合培养液进行代谢,生产短梗霉多糖。

Pullulan is a kind of microbial exopolysac-chafide with important applicable value. Starch waste water is an inevitable byproduct during starch production with complicate components and rich nutrients. Malt root is the by-product of malt manufacture, rich in amylase, the malt enzyme, the tartaric acid enzyme and the proteinase, richly contains the B race vitamin, and includes the massive unknown growth factor. Therefore, enzymolysis of starch industrial waste water with malt root including rich enzyme and the growthfactor, is advantageous for the growth and metabolism ofAureobasidium pullulans, the experiment indicated, it has the certain feasibility. The optimum conditions were at temperature of 28-30℃, 180 r/min, initial pH 6.5 and fermentation time 120 h. Comparatively suits to the metabolism ofAureobasidium pullulans with the mix nutrient fluid of the malt root and the starch waste water, produces the pullulan polysaccharide.