成熟中国鲎组织中的蛋白质与氨基酸含量分析

Analysis of Protein and Amino Acid Content of Different Tissues in Mature Tachypleus ttridentatus

为充分利用资源,采用凯氏定氮法和反相液相色谱法分别测定了成熟中国鲎肌肉、胃、肠道和生殖腺等组织中蛋白质和氨基酸的含量。结果表明:中国鲎肌肉、胃、肠道和生殖腺中蛋白质含量分别为66.79%、62.07%、58.28%和61.94%;随组织类型不同,氨基酸组成模式显著不同;各组织中人体必需氨基酸含量之和分别占其氨基酸总量的50.32%、43.90%、47.37%和55.95%。

In order to make the best use of resource, protein and amino acids content in muscles, stomach, intestine and reproductive gland of mature Tachypleus tridentatus were determined with Kieldahl method and RT-HPLC method. The results show that protein content in muscle, stomach, intestine and reproductive gland are 66.79%, 62.07%, 58.28% and 61.94% respectively; amino acid composition mode differs from tissue. The total content of essential amino acids in muscle, stomach, intestine and reproductive gland of Tachypleus tridentatus are 50.32%, 43.90%, 47.37% and 55.95% of total amino acids in the correspoding tissue.