荧光光度法测定鱼肉中的氧氟沙星残留

Determination of Ofloxacin Residues in Fish Musculature by Spectrofluorimetry

采用荧光光度法测定了鲫鱼、鳗鱼、鲤鱼和罗非鱼肌肉组织中的氧氟沙星药物残留量。样品用酸性乙腈提取,正己烷脱脂,水相在B-R(pH4.0)缓冲溶液中,激发波长λex294nm,荧光波长λem496nm下用荧光光度法测定其中的氧氟沙星含量,氧氟沙星的线性范围0.03～0.20μg/ml,相关系数r=0.9929,检出限为0.005μg/ml。回收率为76.0%～94.5%。日内变异系数为2.14%～6.50%,日间变异系数为3.02%～9.78%。

The spcctrofluorimetfic method is described for assaying ofloxacin in musculature of crucian, eel, carp and tilapia. The ofloxacin in fish samples was extracted with acidic acetonitrile and defatted with hexane. The aqueous phase was determined by spectrofluorimetry using Briston-Robinson buffer solution（pH 4.0） and detected at λex294nm, λem496nm. The linear range of the concentration of ofloxacin is 0.03-0.20 la g/ml with r=0.9929. The limit of detections（LOD） is 0.005 μg/mL The recoveries of ofloxacin is 76.0% to 94.5%. The variation coefficient intra assay is 2.14% to 6.50% and inter assay is 3.02% to 9.78%