叶黄素酯在体内消化吸收过程中水解的研究

Hydrolysis of Lutein Ester during Its Digestion and Absorption in vivo

叶黄素已经证实具有对视网膜黄斑衰退症的防治作用,被认为是一种在食品或药品中有前景的功能因子。叶黄素的制备原料为万寿菊的花,并以叶黄素酯的形式存在。在本研究中,首先从万寿菊干花颗粒中制备叶黄素酯,然后与食用油配制成灌胃液,给SD雄性大鼠一次灌胃,2h后采血,萃取其中的类胡萝卜素,HPLC分析萃取物。分析结果显示:在血清中只能检测到叶黄素单体。这个结果证实:叶黄素酯在消化和吸收过程中被水解成单体,客观上证明了在功能性食品和药品中直接应用叶黄素酯的可能性。

Lutein was proved to have macular degeneration （MD） protection and therapy activities and therefore considered as a functional factor applied in food and pharmaceutical products with a bright future. Lutein was prepared from the flower of marigold. In the flower, lutein exists in ester form. In this study, lutein esters were prepared from marigold flower and then mixed with dietary oil. SD rat was fed with the mixture by gastric administration. After 2 h, blood was collected. Carotenoids were extracted from the blood for HPLC assay. Data from HPLC analysis suggested that only lutein monomer could be observed. This observation proved that lutein esters were hydrolyzed during its digestion and absorption. This conclusion proves a possibility to apply lutein esters in functional food and medicines directly.