盐酸小檗碱对亚硝酸盐中毒小鼠脑急性缺氧的保护作用

Protective Effect of Berberine on Acute Hypoxia Induced by Sodium Nitrite in Mice

目的观察盐酸小檗碱对小鼠脑急性缺氧的保护作用。方法利用亚硝酸钠(NaNO2)中毒和断头实验复制缺氧模型,用盐酸小檗碱3个剂量组即2.0mg/kg、4.0mg/kg、8.0mg/kg连续灌胃6d,观察小鼠存活时间、断头张口次数和喘息时间;用试剂盒测量脑组织超氧化物岐化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)和乳酸脱氢酶(LDH)含量。HE染色观察脑组织形态学变化。结果盐酸小檗碱能明显延长急性缺氧条件下小鼠的存活时间(P<0.05,P<0.01)、断头喘息时间(P<0.05,P<0.01),提高缺氧小鼠脑组织SOD和LDH的活性,减少MDA和NO的含量;镜下可见模型组小鼠脑膜出血,皮质细胞坏死,脑水肿;盐酸小檗碱治疗组小鼠除脑膜血管扩张外未见明显异常。结论盐酸小檗碱对亚硝酸盐中毒小鼠脑急性缺氧具有一定的保护作用。

Objective To investigate the protective effect of berberine on acute hypoxia induced by sodium nitrite （NaNO2） in mice. Methods The animal model of acute hypoxia was established with NaNO2. Then the mice were treated with berberine by garage in three different doses of 2.0 mg/kg, 4.0 mg/kg, and 8.0 mg/kg once everyday respectively for 6 days. The survival time according to the last breath, the breath time and open mouth times of animals were recorded. The superoxide dismutase （SOD）, maleic dialdehyde （MDA）, nitric oxide （NO） and lactic dehydrogenase （LDH） in brain tissue were tested. The pathological change was examined by HE staining. Results Berberine could significantly prolong the living time of acute hypoxia mice induced by NaNO2 and breath time after decapitation （ P〈0.05, P〈0.01）, increase the activity of SOD and LDH, decrease the content of MDA and NO in brain tissue of hypoxia mice （P〈0.05, P〈0.01）. Under microscope, there were meningorrhagia, cellular necrosis in brain tissues of model animals, but no pathological changes found in berberine-treated animals. Conclusion Berberine has certain protective effect on acute hypoxia induced by NaNO2 in mice.