郁金对低张性缺氧小鼠脑功能的影响

Effect of Yujin on Cerebral Functions of Acute Hypoxia Mice

目的观察郁金对低张性缺氧小鼠脑功能的影响。方法利用常压密闭耐缺氧实验和断头实验复制小鼠急性缺氧模型，用郁金低、中、高剂量（10g／kg、20g／kg、40g／kg）连续灌胃6d。采用低张性缺氧模型观察各组小鼠存活时间；通过断头实验观察各组小鼠张口次数和喘吸时间；检测脑组织超氧化物岐化酶（SOD）活性和丙二醛（MDA）含量；镜下观察脑组织形态变化。结果郁金高、中剂量组小鼠急性缺氧条件下的存活时间显著延长（P〈0．001），各郁金剂量组小鼠断头喘息时间、断头后张口次数均增加（P〈0．05～0．001），脑组织SOD活性显著提高（P〈0．001），MDA含量明显下降（P〈0．01～0．001）；镜下可见模型组小鼠脑组织有明显的嗜神经现象、血管周围炎性水肿和脑膜下出血，而郁金治疗组小鼠脑组织除偶见脑膜血管扩张外未见其他异常。结论郁金对低张性缺氧小鼠脑组织有一定的保护作用。

Objective To observe the effect of Yujin on cerebral function of acute hypoxia mice. Methods The acute hypoxia mice model was reproduced by cutting heads and establishing normal-pressure and closed hypoxic experimental settings. In yujin groups, the mice were treated with Yujin at the doses of 10 g/kg, 20 g/kg, 40 g/kg respectively through the intragastric tubes everyday. 6 days later, the mice survival time, the open-mouth times after cutting heads and the breathing time were observed. The activity of superoxide dismutase （SOD） and content of maleic dialdehyde （MDA） in cerebral tissues were measured. The morphological changes of the cerebral tissues under the microscope were examined. Results Yujin could significantly prolong the survival time （P 〈0. 001） and the breathing time and increase the open-mouth times （ P 〈0.05～0. 001）, increase the activity of SOD （ P 〈0. 001） and decrease the content of MDA （P 〈0.01～0. 001） in cerebral tissues. Compared with control group, the Yujin group mice had no obvious pathological alteration except some cerebral vascular dilatation. Conclusion Yujin has an obvious protective effect on cerebral tissues of acute hypoxia mice.