舒血宁注射液对大鼠局灶性脑缺血的保护作用

Protection of Shuxuening Injection on Focal Cerebral Ischemic in Rat

目的探讨舒血宁注射液对局灶性脑缺血引起的脑水肿、神经学行为及脑组织生化指标的影响。方法90只Wistar大鼠随机分为假手术组、模型组、舒血宁1组、舒血宁2组和舒血宁3组，每组18只。采用阻断一侧大脑中动脉制作局灶性脑缺血模型，通过肌肉注射不同剂量的舒血宁注射液（舒血宁1～3组分别为1mg／kg、3mg／kg、10mg／kg；模型组注射生理盐水），各组动物预先给药3d，每日2次，并于造模前0．5h、造模后4h、8h各给药一次，观察各造模组动物在阻断大脑中动脉24h后，神经功能、脑梗死体积及脑组织含水量的变化，测定脑组织中乳酸（LA）、丙二醛（MDA）、超氧化物岐化酶（SOD）含量并与假手术组进行比较分析。结果阻断一侧大脑中动脉后，动物神经功能出现明显变化，阻断侧大脑半球严重水肿。舒血宁注射液剂量依赖性地降低脑梗死体积及阻断侧大脑半球的含水量，改善神经功能，增高脑组织SOD活性，降低MDA、LA含量。结论较大剂量的舒血宁注射液（≥3ml／kg）对局灶性缺血脑组织有保护作用。

Objective To explore the effects of Shuxuening injection on cerebral edema and changes of neurological function induced by focal cerebral ischemia. Methods 90 Wistar rats were randomly divided into the sham operation group, model group, Shuxuening 1st group, Shuxuening 2nd group and Shuxuening 3rd group with 18 animals in each group. The model of focal cerebral ischemia was established by the middle cerebral artery occlusion （MCAO）. Shuxuening of different doses was infused into muscle respectively in the different group （1 mg/kg in Shuxuening 1st group, 3 mg/kg in Shuxuening 2nd group, 10 mg/kg in Shuxuening 3rd group; the model group infused with saline）. The changes of the neurological function, water content of brain tissue and volume of cerebral infarction were determined 24 hours after cerebral ischemia. The activity of lactate （LA）, malondialdegyde （MDA）, superoxide dismutase （SOD） in the brain tissue were measured. Results After MCAO, the disturbance of neurological function and severe cerebral edema were found in model rats. The water content of brain tissue and volume of cerebral infarction decreased, neurological function improved, the activity of SOD significantly increased, and the contents of LA and MDA significantly decrease after Shuxuening injection with a dose-dependent change. Conclusion The Shuxuening injection （≥3 ml/kg） presents a protective effect on brain tissue with focal cerebral ischemia.