东莨菪碱对吗啡依赖大鼠条件位置性偏爱激活的抑制作用

The Inhibition Efficacy of Scopolamine on Reactivation of conditioned place preference in Morphine-Dependent Rats

目的:观察东莨菪碱(Scopolamine,Sco)对吗啡(Morphine,Mor)依赖大鼠条件位置性偏爱激活的抑制作用。方法:以剂量递增连续皮下(SC)给吗啡6天建立吗啡诱导大鼠条件位置性偏爱(CPP)模型,第7天用生理盐水替代吗啡训练大鼠10天,使形成的CPP逐渐消退,单次SC4mg/kg吗啡激发消退的CPP。部分大鼠在注射吗啡前30分钟分别腹腔注射(ip)Sco(1mg/kg、2mg/kg、3mg/kg)。观察东莨菪碱对吗啡依赖大鼠在伴药箱停留时间的变化。结果:与Mor依赖组相比在SC4mg/kgMor引燃刺激前30min应用Sco1mg/kg、2mg/kg、3mg/kg均可以使大鼠在伴药箱停留时间缩短(P<0.05)。结论:Sco一定程度上抑制Mor引燃的Mor依赖大鼠的条件位置性偏爱激活。

Objective： To study inhibitory efficacy of scopolamine on reactivation of conditioned place preference（CPP） in morphine dependent rats. Methods： Morphine was administered by subcutaneous injection at gradually increasing dose （from10mg/kg to 60 mg/kg） for 6 days to establish morphine conditioned place preference. From day 7, the rats were administered normal sodium instead of morphine for 10 days to induce CPP extinction. The rats were given a single priming injection of morphine 4 mg/kg to reinstate the morphine CPP, some rats were treated by ip scopolamine （1mg/kg, 2mg/kg, 3mg/kg） prior to priming injection of morphine. Results： After priming injection of morphine 4 mg/kg, the time spent on the drug paired side was significantly reduced because of the treatment with scopolamine, compared with morphine group （P〈0.05）. Conclusion. Scopolamine has some inhibitory effects on reactivation of conditioned place preference in morphine-dependent rats.