球形羟基磷灰石纳米颗粒的可控合成及其对间充质干细胞生长分化的影响

Controllable Synthesis of Spherical Nano Hydroxyapatites and Their Effects on Growth and Osteogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cells

本文采用CTAB为添加剂进行球形纳米相羟基磷灰石(nHAP)的可控合成,并采用透射电子显微镜(TEM)、X-射线衍射仪(XRD)、傅立叶变换红外光谱仪(FTIR)和精密接触角测量仪对所制得的纳米颗粒的物性进行了表征。结果表明所制得的纳米颗粒为部分结晶的羟基磷灰石,颗粒为均匀球形,粒径约为20nm,具有很好的亲水性。由该纳米颗粒构成的生长基质有利于骨髓间充质干细胞的贴壁、增殖以及成骨分化,是一种良好的骨组织工程支架材料。

The spherical hydroxyapatite nanoparticles （nHAP） were synthesized by using surfactant hexadecyl（cetyl）trimethyl ammonium bromide（CTAB） as surface modifier and stabilizer. The physical-chemical properties of nHAP were charactered using TEM/SAED,XRD,and contact angle measurements. The different effects on growth and osteogenic differentiation of bone marrow derived mesenchymal stem cells （BMSCs） between the nHAP particles and the conventional HAP crystallites （cHAP） were studied. The results showed that the nHAP particles were more uniformed in shape and size and therefore,its thin films presented smoother and better-formed surface. BMSCs seeded on nHAP thin films showed enhanced attachment and proliferation,increased osteogenic marker expression such as alkaline phosphates （ALP） and osteocalcin （OC） compared with those on the cHAP thin films.