摘要
目的研究转化生长因子-β1(TGF-β1)、Smad2、Smad4在羊驼睾丸的表达与定位,探索TGF-β1在羊驼睾丸发育及精子发生中的作用。方法以24月龄的羊驼睾丸为研究对象,采用Western blotting技术及免疫组织化学SABC法对TGF-β1、Smad2、Smad4在羊驼睾丸的表达进行研究。结果Western blotting结果显示,羊驼睾丸组织粗蛋白提取物中存在分子量约为25kD5、2kD、62kD,与多克隆兔抗TGF-β1、Smad2、Smad4抗体发生免疫阳性反应的蛋白条带;免疫组织化学结果显示,TGF-β1、Smad2、Smad4在24月龄羊驼睾丸的间质细胞、支持细胞和各级生精细胞均有不同程度的表达。结论TGF-β1通过Smad2与Smad4传导途径参与性成熟羊驼睾丸组织的发育与精子发生的调控。
Objective To study the expression and localization of TGF-β1 and its signal transducer Smad2 and Smad4 which play important roles during testicular development and spermatogenesis in alpaca testis. Methods The whole testes were obtained from alpacas aged 24 months （ n = 3 ） at the Scientific Research Base of Shanxi Agriculture University. The protein expressions of TGF-β1,smad2 ,smad4 in alpaca testes were examined by Western blotting and SABC. Results The distributions of TGF-β1 ,Smad2 and Smad4 in the testes of alpacas aged 24 months were demonstrated. Conclusion Our findings show that TGF-β1, Smad2 and Smad4 are involved in the regulation of spermatogenesis, and provide direct evidences for the moleculer mechnism of TGF-β1 action in the alpaca testis during spermatogenesis.
出处
《解剖学报》
CAS
CSCD
北大核心
2007年第4期481-485,共5页
Acta Anatomica Sinica
基金
引进国际先进农业科学技术计划(948计划)(202096)(2003-Z86)提供资助
