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马兜铃酸对人肾小管细胞分泌性磷脂酶A_2的影响 被引量:2

Investigation of Aristolochic Acid on Secretory Phospholipase A_2 of Human Kidney Cell in Vitro
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摘要 目的:研究马兜铃酸(aristolochic acid,AA)对人肾小管细胞(human kidney cell,HKC)的损伤与分泌性磷脂酶A2(secretory phospholipase A2,sPLA2)活性的影响。方法:以体外培养的HKC为研究对象,脂多糖(LPS)和氯化钙(CaCl2)为sPLA2激活剂,将实验分为单用AA组和联用激活剂组两大类,用sPLA2活性检测试剂盒检测上清中sPLA2活性改变。结果:10 mg/L AA刺激HKC 24 h后,引起细胞上清sPLA2活性降低(1.154±0.079 vs 1.389±0.123),与正常对照组比较无统计学意义。随着AA剂量的增大,sPLA2活性升高,40 mg/L时高达5.422±0.091(P<0.05),且40 mg/L AA培养组sPLA2活性表现出与时间呈正相关的变化。2LPS和CaCl2剂量正相关地激活HKC sPLA2,该过程可为10 mg/L AA所抑制,呈浓度依赖性。结论:10 mg/L AA可抑制LPS和CaCl2激活HKC sPLA2,呈浓度正相关性。同时,较高浓度AA可损伤HKC激活sPLA2。据此,推测AA损伤HKC的过程中,sPLA2活性达不到损伤程度应有的水平。 Objective:To investigate the effect of aristolochic acid on secretory Phospholipase A2 (sPLA2) activity of human kidney cells (HKCs) in vitro. Methods: HKCs were cultured with aristolochic acid (AA) and/or sPLA2 agonists including lipopolysaccharide (LPS) and CaCl2. sPLA2 activity in the supernatant of HKCs was determined with sPLA2 assay kit. Results:Compared with the normal group, sPLA2 activity in the supernatant of 10 mg/L group decreased slightly( P 〉0.05 )while sPLA2 activity increased markedly in AA 20 mg/L and 40 mg/L treated HKC groups. There was positive correlation between sPLA2 activity and AA concentration ( 10-40 rng/L, r = 0. 923, P 〈 0.01 ), or cultured time (40 mg/L AA, 12 h-48 h, r = 0. 797, P 〈 0.01 ) significantly. 2. AA inhibited sPLAz activated by 1 200 μmml/L CaCIz or 1 200 ng/ml LPS in a concentration - dependent manner. ( r = 0. 765, P〈 0.01 and r = 0. 936, P〈 0.01, repectively). Conclusion: AA inhibited sPLAz activated by LPS or CaCl2 in a concentration- dependent manner. In the meantime, AA (concentration≥20 mg/L) could damage sPLA2 activiation by HKC. So we concluded that, sPLA2 activity cannot reach what the damage should have, when there is an aristolochic acid nephropathy.
出处 《中国中西医结合肾病杂志》 2007年第8期458-461,共4页 Chinese Journal of Integrated Traditional and Western Nephrology
关键词 马兜铃酸 分泌性磷脂酶A2 人肾小管细胞 Human kidney cell Secretory Phospholipase A2 Aristolochic acid
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