转基因西洋参冠瘿组织中多糖组分提取条件的优化考察

Optimization of Extraction Methods of Polysaccharides Constituents from the Crown Gall Cultures of Panax quinquefolium

目的优化考察转基因西洋参冠瘿组织中多糖提取条件。方法用正交设计法优选西洋参冠瘿组织中多糖的最佳提取工艺,选取提取温度、加水量、提取时间和提取次数四个考察因素。结果正交实验结果显示:西洋参冠瘿组织中多糖的最佳提取工艺:提取温度为70℃,40倍溶剂,提取3次、3 h/次。结论优选得到的工艺简单易行,稳定性好。

Objective To investigate the extraction methods of polysaccharides constituents from the crown gall cultures of Panax quinquefoliurns. Methods The orthogonal test was employed to optimize and establish the extraction technology of water - soluble polysaccharides from the crown gall of P. quinquefolium. Results When employed 700C of extracting temperature, 40 times of water, refluxing and extracting for 3 times, 3 hours for every turn, the optimal extraction efficacy was obtained. Conclusion Extraction method optimized will be used for the isolation and purification of polysaccharides from the transgenic crown gall cultures of P. quinquefolium.