高效液相色谱法测定首乌精口服液中二苯乙烯苷的含量

HPLC Content Determination of Stilbenes in Shouwujing Oral Solution

目的建立首乌精口服液中2，3，5，4’四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量的高效液相色谱（HPLC）测定方法。方法色谱柱为Waters公司Symmetry—Ci8柱（3、9mm×250mm，5μm），流动相为乙腈-水（17：83），流速1．0ml／min。检测波长为320nm。结果2，3，5，4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷在0．45～1．8μg范围内线性关系良好（r=0．999987），8h的RSD为0、7％。结论该方法量测首乌精口服液中有效成分二苯乙烯苷的含量，灵敏度高、定量准确、方法简便、重现性好，能有效地控制首鸟精口服液制剂质量。

Objective An HPLC method was established for the determination of 2,3,5,4＇-tetrahydroxystilbene-2-O-13-D-glucopyranoside in Shouwujing Oral Solution. Methods A Symmetry- C18 column（3.9 mm×250 mm,5 μm）with a mobile phase composed of acetonitrile-water（ 17：83 ）was used. The detection wavelength was 320 nm and the flow rate was 1.0 ml/min . Results The calibration curves were linear between 0.45 - 1.8μg （ r = 0. 999 9）, the 8 hours＇precision RSD was 0.7% . Conclusion The method is simple, rapid and accurate and is suitable for the determination of 2,3,5,4＇-tetrahydroxystilbene-2-O-β-D-glucopyranoside in Shouwuiing Oral Solution.