新疆软紫草中咖啡酸四聚体对照品的制备

Preparation of Caffeic Acid Tetramers Reference Substance from Arnebia euchroma

目的从新疆软紫草的丙酮水提取物中分离制备了咖啡酸四聚体对照品。方法经紫外光谱、红外光谱、核磁共振和质谱确定结构。结果其相关数据与文献一致,纯度为98.11%。结论符合中药化学对照品含量测定要求,可作为高纯度咖啡酸四聚体化学对照品的制备分离方法。

Objective To prepare reference substance for quality control of Arnebia euchroma. Caffeic acid tetramers was extracted and separated from acetone water extraction of Arnebia echroma. Methods Chemical structure of the caffeic acid tetramers product was elucidated by ultraviolet spectrometry（UV） ,infrared spectroscopy（ IR）, nuclearmagnetic resonance（NMR） and mass spectrometry（MS）. Results The results were in agreement with the reference. The purity of the caffeic acid tetramers product was 98.11%. Conclusion The method meets the reference substances of traditional Chinese medicines and can used to prepare and purify the active constituents from Arnebia euchroma.