高效液相色谱法测定更年乐胶囊中淫羊藿苷的含量

Content Determination of Icariine in Gengnianle Capsule by HPLC

目的建立更年乐胶囊中淫羊藿苷的含量测定方法。方法采用高效液相色谱法，以C-s色谱柱（5μm，4．6mm×200mm）；乙腈：水（30：70）为流动相；流速1．0ml／min，检测波长270nm。结果淫羊藿苷在0、204～0、816μg范围内呈良好的线性关系，r=0．9999。平均回收率为98．77％，RSD为1．58％。结论该方法简单、灵敏度高，可用于更年乐胶囊中淫羊藿苷的含量测定。

Objective To establish HPLC method for the determination of Icariine in Gengnianle Capsule. Methods LUNA ODS C18 BDS （200 mm ×4.6 mm） was used with acetonitrile water （30： 70） as a mobile phase. The flow rate was 1.0 ml·min^-1. The detecting wavelength was 270 nm. Results The linear range of Icariine was 0.201×0.816 μg,r =0. 999 9. The average recovery was 98.77%, Conclusion The method is simple ,rapid and accurate. Key words ：