人参多糖对HCG诱导黄体与颗粒细胞功能的影响

The Effect of Ginseng Polysugar on Secretion Function of Luteal and Granulose Cell of Rat in vitro

目的研究人参多糖对大鼠卵巢功能的作用。方法采用体外细胞培养观察人参多糖对HCG诱导的黄体与颗粒细胞分泌孕酮与cAMP的影响,并检测了卵母细胞的存活率。分离培养黄体、颗粒与卵母细胞,设对照组与不同浓度人参多糖实验组,采用放免分析方法测定孕酮与cAMP生成含量,细胞存活率检测采用MTT法。结果与对照组比较,孕酮含量:黄体细胞为(6573.46±185.14)与(4509.55±126.82)pmol,颗粒细胞为(197.16±42.87)与(320.42±12.46)pmol。cAMP:黄体细胞为(31.20±17.13)与(65.26±15.93)fmol,颗粒细胞为(121.15±19.96)与(296.42±27.28)fmol。结论人参多糖抑制HCG诱导黄体细胞孕酮分泌,促进HCG诱导颗粒细胞孕酮分泌。而协同黄体细胞与颗粒细胞cAMP生成,人参多糖使卵母细胞生长抑制率降低,呈区间剂量依赖关系。

Objective To study using of ginseng polysugar on secretion function of female rat ovary. The experiment had observed progesterone and cAMP by HCG inducing luteal and granulose cell as well as living cell rate of oocyte in vitro. Methods Rat luteal and granulose cell and oocyte was cultivated, cell counting was worked by. microscope, progesterone and cAMP content was determined by radioimmunoassay kit（ RIA kit）. Cell exist rate of oocyte was determined by MTF way, Results Progesterone： the luteal cell test groups compare with control group, 6573.46 ± 185.14 and（4509.55 ±126.82） pmol, The granulose cell test groups compare with control group, 197.16±42.87 and （320.42±12,46）pmol. cAMP： the luteal cell test groups compare with control group, 31.20±17.13and（65.26±15.93） fmol,the granulose cell test groups compare with control group, 121.15 ±19.96 and （296.42 ± 27.28）fmol, Conclusion This treatise had demonstrated that ginseng polysugar can inhibit HCG inducing progesterone secretion and enhancing cAMP content of luteal and granulose cell of rat, ginseng polysugar has lower oocytes cell grow up inhibitory rate. It is evident that ginseng polysugar possesses characteristic of dose dependent.