摘要
目的采用荧光分光光度法，建立了大黄中游离蒽醌类成分含量测定的方法。方法以丙酮为溶剂，在hex=460nnq和kern=540nnq处测定荧光强度。结果测得游离蒽醌含量在o．25～3．oμg／ml范围内呈良好的线性关系，线性回归方程为F=122．81C＋46．224（r=0．9996），最低检测限为0．25μg／ml，表明该法灵敏度高、重现性较好，且操作简便。结论该研究为测量大黄中活性成分游离蒽醌提供了一种有效可靠的分析方法。
Objective With the fluorescence spectrophotometry,to build up free anthraquinones in Rheum emodi method of Assay. Methods Take acetone as solvent agent, with the Aex =460 nm and Aem = 540 nm determination fluorescence strength. Resuits Free anthraquinone contents were in 0.25 - 3.0 of μg/ml with good linear relationship. The linear equation F = 122.81C ＋ 46.224（ r =0. 999 6） , the lowest detectability was 0.25μg/ml. The method was sensitive and reproducible. Conclusion This research for measuring live composition of free anthraquinone in Rheum emodi Wall provides a kind of effectively dependable analytical method.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2007年第8期1939-1940,共2页
Lishizhen Medicine and Materia Medica Research
关键词
大黄
游离蒽醌
荧光分光光度法
Rheum emodi
Free anthraquinone
Fluorescence spectrophotometry
