超临界CO_2萃取三七脂溶性成分及其GC-MS分析

Supercritical CO_2 extraction of lipophilic fraction from Panax notoginseng and its GC-MS analysis

目的:用超临界CO2萃取三七的脂溶性化学成分,分析并测定提取物的主要成分及其含量。方法:使用均匀设计对影响超临界CO2萃取的各因素进行考察优化;萃取物用气相色谱-质谱联用法进行分析鉴定,使用面积归一法测定各成分相对含量,并与乙醚溶剂直接提取法进行对比。结果:萃取最佳条件为:萃取釜压力30MPa,萃取温度48℃,分离釜Ⅰ压力8MPa、分离温度30℃,分离釜Ⅱ压力5MPa、分离温度30℃,萃取时间2.0h,CO2流量35kg.h-1,三七颗粒度60目;经气相色谱-质谱联用法分析,超临界CO2萃取物与乙醚溶剂直接提取物的组成有显著不同,检出多种炔醇类成分,包括人参炔醇(5.95%)。结论:用超临界CO2萃取三七,可以较好的获得其脂溶性成分,提取物可以供进一步深入研究。

OBJECTIVE To study the optimum condition of extracting lipophilic fraction of Panax notoginseng by supercritical CO2 extraction （SFE） and analyze its chemical constituents. MET＇dOllS The SFE technology was chosen and optimized by an even-designed method. The chemical constituents of SFE were separated and identified by gas chromatography-mass spectrometry（GC-MS）, and their relative contents were determined by normalization method of area. The chemical constituents of SFE were compared to conventional solvent extraction. RESULTS The optimum technological conditions were as follow： extracting pressure 30 MPa,extracting temperature 48 ℃ separator Ⅰpressure 8 MPa, separator Ⅰtemperature 30 ℃ ; separator Ⅱpressure 5 MPa, separator Ⅱtemperature 30℃. extraction period 2 hours;and CO2 flow rate 35 kg·h^-1. The chemical constituents of SFE were significantly different from conventional solvent extraction. There were more polyacetylenes in SFE than conventional solvent extraction, including panaxynol （5.95%）. CONCLUSION The SFE technology used for extracting lipophilic fraction from Panasc notoginseng is better than the conventional solvent extraction.