大黄素对原代培养小鼠肝细胞非酒精性脂肪变的影响

Effects of emodin on nonalcoholic fatty degeneration of primary cultured murine hepatocytes

[目的]观察大黄素对原代培养小鼠肝细胞非酒精性脂肪变的影响。[方法]采用改良的胶原酶原位灌流法分离小鼠肝细胞,以含50%胎牛血清的1640培养液造成肝细胞脂肪变,加入不同浓度大黄素,检测培养上清液肝酶活性并观察细胞形态学变化。[结果]以50%胎牛血清的1640培养液培养的肝细胞明显脂肪变性,大黄素干预组培养上清液丙氨酸氨基转移酶(ALT)、门冬氨酸转氨酶(AST)、谷氨酰转肽酶(GGT)、乳酸脱氢酶(LDH)活性较模型组显著降低,苏木精-伊红染色显示肝细胞脂肪变性减轻。[结论]大黄素可明显减轻原代培养小鼠肝细胞的脂肪变性。

[Objective]To investigate the effects of emodin on nonalcoholic fatty degeneration of primary cultured murine hepatocytes. [Methods]Primary murine hepatocytes were isolated by collagenase infusion method in situ. Nonalcoholic hepatosteatosis was induced by 50% inactivated embryo blood serum. Simutaneously emodin of different concentration was added into cultured medium. The enzyme activity in cultured medium and celluar morphology were observed.[Resuits] Nonalcoholic fatty degeneration model was established successfully with 50% inactivated embryo blood serum. The activity of alanine aminotransferase（ALT）,aspartate aminotransferase （AST） .gamma glutamyl transferase （GGT）, lactate dehydrogenase （LDH）in cultured medium were decreased significantly after the treatment of emodin. HE staining showed that hepat osteatosis was alleviated. [Conclusion ]Emodin can alleviate nonalcoholic fatty degeneration of primary cultured murine hepatocytes.