银苓Ⅰ号对铅处理Vero细胞活力和氧化损伤的影响

Effects of YinlingⅠon Cell Viability and Oxidative Injury of Lead-Exposed Vero Cell

目的研究中药复方银苓Ⅰ号对铅处理Vero细胞活力和氧化损伤的影响作用。方法通过细胞活力、超氧化物歧化酶(SOD)及丙二醛(MDA)测定,观察银苓Ⅰ号对铅处理Vero细胞活力和氧化损伤的影响。结果Vero细胞暴露在125μmol/L醋酸铅环境中时,细胞活力显著降低,细胞培养液中SOD活性下降、MDA量增加;用不同质量浓度银苓Ⅰ号干预后,细胞活力、细胞液SOD活力均升高,MDA量降低。结论银苓Ⅰ号对铅处理Vero细胞活力和细胞氧化损伤具有保护作用,且有一定剂量依赖关系。

Objective To study effects of yinling Ⅰ on cell viability and oxidative injury of lead - exposed cell. Methods After lead exposure Veto cell was treated with yinling Ⅰ of different concentrations. The cell viablitity was measured by methyl thiaxiolyl tetrazolium（MTT） method and the superoxide dismutase （SOD）and malondialdehyde（MDA） activity in cell suspensions were measured. Results When Veto cell lived in 125 μmol/L lead acetate surrounding,the MDA concentration increased,but the viability of cell and the SOD content in the Veto cell suspension decreased. Yinling Ⅰ could increase the viability of lead- exposed cell during a certain extent;in the most non- toxicity concentration yinling Ⅰ could elevate the SOD content in the Veto cell suspension ,reduced the MDA concentration and resisting lead toxieation in vitro. Condusion Yinling Ⅰ has the protective effects on the cell viability and oxidative iniurv of lead - exposed cell.