人参果皂苷注射液对实验性高血糖的影响

Effect of Injection of Ginseng Fruit Saponins on experimental hyperglycemia

目的:研究人参果皂苷注射液(IGFS)的降血糖作用。方法:①以1.5%四氧嘧啶造成大鼠实验性高血糖模型后,IGFS按7.5、15.0、30.0mg·kg-1剂量给大鼠腹腔注射14d,测定IGFS治疗性给药后空腹血糖(FBG)、肝糖原和血清丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性;②IGFS按10、20、40mg·kg-1剂量给小鼠腹腔注射7d,以0.1%肾上腺素建立小鼠实验性高血糖模型,测定动物的FBG及肝糖原含量;③IGFS按7.5、15.0、30.0mg·kg-1剂量给正常大鼠腹腔注射7d,观察FBG、血清胰岛素水平及C-肽含量,计算胰岛素敏感指数(ISI)。结果:与模型组比较,IGFS7.5、15.0、30.0mg·kg-1治疗性给药14d,四氧嘧啶性高血糖大鼠FBG及血清MDA含量降低(P<0.05),肝糖原含量及SOD活性明显提高(P<0.01);与模型组比较,IGFS20.0、40.0mg·kg-1预防性给药7d,肾上腺素性高血糖小鼠FBG明显降低(P<0.01),肝糖原含量明显升高(P<0.05);IGFS7.5、15.0、30.0mg·kg-1预防性给药7d,正常大鼠血清C-肽含量比对照组明显增加(P<0.05或P<0.01),但对FBG、血清胰岛素含量及ISI均无明显影响。结论:IGFS对四氧嘧啶及肾上腺素所致的实验性高血糖均具有明显降糖作用,可能与其清除自由基、抑制脂质过氧化及增加葡萄糖的转化和利用有关。

Objective To study the hypoglycemic activity of Injection of Ginseng Fruit Saponins （IGFS） . Methods Fasting blood glucose （FBG）, the contents of hepatic glycogen and the serum malondialdehyde （MDA） and activity of the serum super oxide dismutase （SOD） were determined in hyperglycemia rats induced by alloxan 14 d after administration of IGFS （in dosages of 7.5 - 30.0 mg·kg^-1 intraperitoneal injection）. FBG and the content of hepatic glycogen were determined in hyperglycemia mice induced by adrenaline 7 d after administration of IGFS （in dosages of 10-40mg·kg^-1 intraperitoneal injection）. At the same time, FBG and contents of the serum insulin and C-peptide were also observed, and insulin sensitive index （ISI） was calculated in normal rats pretreated with IGFS （in dosages of 7.5-30.0 mg·kg^-1 intraperitoneal injection）. Results In hyperglycemia rats induced by 1.5% alloxan with sublingual vein injection, compared with normal group, FBG and content of the serum MDA in model group were increased significantly （P〈0.05）, while the content of hepatic glycogen and activity of the serum SOD were decreased （P〈0.05） ;however, compared model group, FBG and content of the serum MDA were decreased significantly （P〈0.01）, the content of hepatic glycogen and activity of the serum SOD were increased （P〈0.05） by IGFS （in dosages of 7.5, 15.0, and 30.0 mg·kg^-1 intraperitoneal injection for 14 d） in hyperglycemia mice induced by 0.1%adrenaline with subcutaneous injection. Compared with model group, FBG was reduced （P〈0.01） and the content of hepatic glycogen was increased （P〈0.05） by IGFS （in dosages of 20. 0 and 40.0 mg·kg^-1 intraperitoneal injection for 7 d） . The content of the serum C-peptide was increased significantly （P〈0.05 or P〈0.01） while FBG and the content of serum insulin and ISI changed little in normal rats pretreated with IGFS （in dosages of 15. 0 and 30. 0 mg·kg^-1 intraperitoneal injection for 7 d）. Conclusion IGFSpossesses the hypoglycemic action on experimental hyperglycemia models induced by alloxan and adrenaline, which be related to scavenging the oxygen free radicals, restraining lipid peroxidation and increasing transformation and utilization of glucose.