兔动脉粥样硬化模型中NO和PAI-1的表达及阿托伐他汀的干预作用

Expressions of nitric oxide and plasminogen activator inhibitor type-1 in rabbit models of atherosclerosis and interference effect of atorvastatin

目的:探讨兔动脉粥样硬化模型中一氧化氮(NO)和血浆纤溶酶原激活物抑制物-1(PAI-1)的表达及阿托伐他汀的干预作用。方法:24只雄性日本大耳白兔随机分为高脂饮食组(A组)、高脂饮食+阿托伐他汀组(B组)和正常饮食组(C组),每组8只。各组分别在喂养16周末处死,ELISA法检测NO和PAI-1。全自动生化分析仪检测血清脂蛋白的表达水平。结果:NO的表达水平B组显著高于A组和C组(P<0.01),A组与C组比较差异无显著性(P>0.05)。PAI-1的表达水平B组低于A组和C组(P<0.01),A组与C组比较差异无显著性(P>0.05)。A、B、C三组总胆固醇(TC)水平分别为(23.51±10.58)、(14.27±3.51)和(1.36±0.33)mmol·L-1,三组间两两比较差异有显著性(P<0.05)。A、B、C三组低密度脂蛋白(LDL)水平分别为(21.39±10.00)、(14.23±4.01)和(0.72±0.35)mmol·L-1,三组间两两比较差异有显著性(P<0.05)。甘油三酯(TG)水平三组间比较差异无显著性(P>0.05)。结论:高脂饮食对兔动脉粥样硬化模型中NO和PAI-1表达水平无明显影响,阿托伐他汀干预使NO表达水平升高,使PAI-1表达水平降低,有利于抑制动脉粥样硬化的进程。

Objective To investigate the expressions of nitric oxide （NO） and plasminogen activator inhibitor type-1 （PAI-1） in rabbit models of atherosclerosis and the interference effect of atorvastatin. Methods Twenty four male Japanese rabbits were divided into 3 groups （8 in each group）： high-fat diet group （group A）, high-fat plus atorvastatin group （group B） and normal diet group （group C）. The expressions of NO and PAI-1 were detected with ELISA assay. Plasma lipid was detected with automatic analysis divice. Results The espression of NO in group B was significantly higher than those in groups A and group C （P〈0.01）; there was no significant difference between groups A and C （P〉0.05）. The expression of PAI-1 in group B was significantly lower than those in groups A and C, there was no significant difference between groups A and group C （P〉0.05）. The levels of serum TC in groups A, B, and C were （23.51±10.58）, （14.27±3.51） and （1.36±0.33） mmol·L^-1, respectively; the levels of serum LDL in groups A, B, and C were （21.39±10.00）, （14.23±4.01） and （0.72±0.35） mmol·L^-1; there were significant differences between three groups （P〈0.05）, while TG had no significant difference between three groups （P〉0.05）. Conclusion High-fat diet has no significant effect on the expressions of NO and PAI-1 in atherosclerosis rabbits . Atorvastatin can increase the expression of NO and decrease the expression of PAI-1, and can inhibit the progression of atherosclerosis.