复方刺五加注射液对药物诱发实验性心律失常的影响

Effects of Compound Acanthopanax Senticosus Injection on experimental arrhythmia induced with medicine

目的:观察复方刺五加注射液(CASI)对药物诱发实验性心律失常的影响。方法:制备氯化钡(BaCl2)、哇巴因及乌头碱诱导的大鼠及豚鼠实验性心律失常模型,CASI按25、50、100mg·kg-1分为3个剂量组,舌下静脉给药1次,不给CASI作为对照组,记录大鼠给BaCl2后心律失常的发生率、出现时间、持续时间及动物的死亡率;观察豚鼠及大鼠出现室性早搏(VP)、室性心动过速(VT)、心室颤动(VF)及心脏停搏(CA)时哇巴因及乌头碱的用量。结果:与对照组比较,CASI25、50、100mg·kg-1组大鼠BaCl2性心律失常的发生率降低(P<0.05),出现时间推迟(P<0.01),持续时间缩短(P<0.05),死亡率减少(P<0.01);CASI50、100mg·kg-1组豚鼠对哇巴因中毒诱发VP、VT、VF及CA的耐受剂量高于对照组(P<0.05或P<0.01);CASI100mg·kg-1组大鼠对乌头碱诱发VP、VT及VF的耐受剂量高于对照组(P<0.05),并且明显优于阳性药维拉帕米注射液。结论:CASI对多种药物诱发的实验性心律失常均具有明显保护作用,其对抗Ca2+性心律失常的作用明显优于Na+性心律失常,提示CASI可能为钙通道阻滞剂。

Objective To observe the effects of Compound Acanthopanax Senticosus Injection （CASI） on experimental arrhythmia induced with medicine in rats and cobayaes. Methods The experimental arrhythmia models were induced with barium chloride （BaCl2）, uabaina and aconitine in rats and cobayaes. The incidence rate and mortality rate of arrhythmia, the emergence time and the duration of arrhythmia induced with BaCl2 were recorded in rats pretreated with CASI （in dosages of 25, 50, and 100 mg·kg^-1 sublingual vein injection）. At the same time, the dosages of uabaina and aconitine were observed when the ventricular premature contraction （VP）, ventricular tachycardia （VT）, ventricular fibrillation （VF） and cardiac asystole （CA） were appeard in rats and cobayaes pretreated with CASI （in dosages of 25, 50, and 100 mg·kg^-1 sublingual vein injection） . Results Compared with control group, the incidence rate of arrhythmia induced with BaCl2 was lowered （P〈0.05）, the emergence time of arrhythmia was delayed （P〈0.01）, the duration of arrhythmia was shortened （P〈0.05）, and mortality rate of arrhythmia was decreased significantly （P〈0.01） in rats pretreated with CASI in dosages of 25, 50, and 100 mg·kg^-1 sublingual vein injection. The tolerance doses of eobayaes on VP, VT, VF and CA induced with uabaina were significantly higher in 50 and 100 mg·kg^-1 CASI groups than control group （P〈0.05 or P〈0.01）, while the tolerance doses of rats on VP, VT and VF induced with aeonitine were also significantly higher in 100 mg·kg^-1 CASI group than control group （P〈0.05）. Conclusion CASI have a significantly protective effect on experimental arrhythmia induced with medicine in rats and cobayaes, its effect on calcium channel is more better than on natrium channel. The results show that CASI may be calcium channel blocker.