雄激素致不孕大鼠子宫转化生长因子β1及其受体的变化

Changes of transforming growth factor β1 and its receptor in uterus in androgen-induced sterile rats

目的:研究雄激素致不孕大鼠(ASR)子宫转化生长因子β1(TGF-β1)及其受体(TGF-βRⅠ)表达量的变化。方法:出生9日龄SD雌性大鼠随机分为ASR模型组(n=12)及对照组(n=10)。模型组大鼠一次性皮下注射丙酸睾丸酮(T)1.25mg,建立ASR模型。正常组注射等量中性茶油。采用放射免疫分析法测定血清T水平,采用免疫组织化学方法检测子宫TGF-β1和TGF-βRⅠ的表达量,采用HE染色观察子宫组织形态学变化。结果:与正常组比较,ASR模型组大鼠血清T水平明显增高(P<0.01);子宫内膜、肌层TGF-β1表达量明显降低(P<0.01);肌层TGF-βRⅠ表达量明显降低(P<0.05),而内膜TGF-βRⅠ表达量却明显升高(P<0.05);与正常组比较,ASR模型组大鼠子宫肌层变薄,内膜萎缩,腺体减少。结论:ASR模型子宫内膜及肌层TGF-β1和TGF-βRⅠ表达量异常,损伤子宫内膜的分化和肌层的发育,影响胚胎的植入。这些变化可能是导致不孕的原因之一。

Objective To observe the changes of transforming growth factor β1 （TGF-β1） and its receptor （TGF-βRⅠ） inthe uterus in androgen-induced sterile rats （ASR）. Methods Twenty two SD female rats of 9-day age were randomly divided into two groups： ASR model group （n= 12） and control group （n= 10）. ASR model was established by injecting testoserone propionate into rats subcutaneously. At the same time, the rats in control group were injected with neutral tea oil with same volume. The level of serum testosterone （T） was determined by means of radioimmunoassay. The expressions of TGF-β1 and TGF-βRⅠ in the uterus were detected by immunohistocbemistry method. The morphologic changes in the uterus by HE staining were observed. Results Compared with control group, the level of serum testosterone in medol group was increased obviously （P〈0. 01）, the expressions of TGF-β1 in the endometrium and myometrium were decreased significantly （P〈 0.01）, the expression of TGF-βRⅠ in myometrium was decreased obviously （P〈0. 05）, while the expression of TGF-βRⅠ in endometrium was increased significantly （P〈0. 05）. In model group , myometrium of the uterus was tenuis, endometrium was atrophic and the number of glandular organs was decreased. Conclusion The expressions of TGF-β1/ TGF-βRⅠ in endometria and myometrium in ASR are abnormal, the differentiation of endonetrium and the growth of myometrium are injuied. These changes might be one of the reasons to lead to infertility.