摘要
YRRM（YchromosomeRNArecognitionmotif）为无精子因子（AZF）的重要候选成分，已证明YRRM的丢失可导致无精子或严重少精。应用PCR方法检测核型正常（46XY）的65例无精症男子和25例严重少精男子基因组DNA中的YRRM，发现6例无精症病人中有YRRM的丢失，严重少精病人中1例有YRRM的丢失。结果表明，应用该方法检测YRRM基因片段是切实可行的，对无精症和严重少精的病因诊断具有一定的应用价值。
YRRM is one of the important candidates for azoospermia factor(AZF). Ithas been proved that the deletion of AZF could cause azoospermia or severe oligozoospermia.In this paper, PCR was used to analyse YRRM gene in 65 azoospermic men and 25 oligozoospermia men with a cytologically normal Y chromosome. YRRM deletion was found in 6azoospermic men. In oligozoospermic men, no deletion was found. The results indicated that PCR assay can be used to analysis YRRM gene which is very useful for clinical diagnosis of azoospermia.
