摘要
应用放射配基结合测定法，以R1881为特异配基；分析正常成年男性雄激素靶组织─—外阴皮肤组织中细胞质和核基质雄激素受体（AR），同时分别用Scatchard、woolf和双倒数作图法三种常用数据处理方法详细分析AR的Bmax（Bm）和Kd。结果表明，作为阳性对照和质量控制的6例雄性大鼠肝脏细胞质AR的Bm为4.77±0．61pmol／g蛋白，Kd为9.662.03×10－10mol／L。11例正常男性的细胞质ARBm为24．03±7．19pmol／g蛋白，Kd为15.13±7.06×10－10mol／L；细胞核内AR的Bm为204．37±119．62pmol／g蛋白，Kd为6．38±4．14×10－10mol／L。Scatchard、Woolf和双倒数法分析的结果均一致。提示AR的Scatchard作图分析是稳定可靠的，并且具有显著的实用价值。
In male, genital skins were the androgen target tissues, which were bountiful of androgen receptor (AR) in cytosol and nucleochylema. Eleven foreskin specimen were taken from the volunteers (2O～34ys) at the time of circumcision. Six rat livers were required just before the receptor assay, as positive and qualitative control. About 1. 0g tissures were homogenized directly, without tissue and cell culture, and cetrifuged to get cytosol. The nucleochylema were extracted with 0. 6 mol/L KCl buffer from the nuclear pellets. The cytosolAR and nuclear AR radioassay were performed with the specific ligand R1881 and 3 methods for analysing data of Scatchard, Woolf and double reciprocal plots. The cytosol AR was characterized by Bm 24. 03± 7. 19 pmol/g protein and Kd (15.13±7. 06)×10-10 mol/L, and nuclear AR, Bm 204. 37±119. 62 pmol/g protein, Kd (6. 38±4.14) ×10-10 mol/L. The resultsof Scatchard, Woolf and double reciprocal plots were identical on statistic (P>0. 05). So the androgen receptor radioassay in the principle of Scatchard pl0ts was stable, reliable and practical.
