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人乳腺癌细胞株KLK5 mRNA的表达及雌激素的调节作用 被引量：3

Expression of KLK5 mRNA in human breast cancer cell line and regulation of estrogen
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摘要 目的探讨雌激素对雌激素受体阳性人乳腺癌细胞株MCF-7和B37组织激肽释放酶5(KLK 5)mRNA的表达水平,以及雌激素对KLK5 mRNA表达水平的影响。方法分别取生长良好的MCF-7和B37细胞,在不同浓度的雌激素(17-βE2)存在下培养72小时后,收集细胞,提取总RNA,采用荧光定量逆转录-聚合酶链反应,检测KLK5 mRNA表达水平及变化。结果在未经刺激的乙醇对照组,MCF-7细胞KLK5 mRNA的相对表达水平(KLK5 mRNA/GAPDH mR-NA)为(3.23±0.51)×10-5,B37细胞KLK5 mRNA表达水平为(3.13±0.62)×10-4。B37细胞KLK5 mRNA表达水平高于MCF-7细胞。当17-βE2浓度10-11mol/L以下时,MCF-7和B37细胞中KLK5 mRNA表达水平与乙醇对照组无明显差异(P<0.05)。随17-βE2浓度的增高,MCF-7和B37细胞中KLK5 mRNA的表达呈递增趋势,各组与乙醇对照组相比差异有显著意义(P<0.01)。结论雌激素对人乳腺癌细胞株MCF-7和B37细胞的KLK5 mRNA表达有剂量依赖的上调作用。 Objective To investigate the effects of estrogen on the expression of KLX5 mRNA in human breast cancer cell line MCF-7 and B37. Methods MCF-7 and B37 cells were incubated with 17-βE2 at different concentration for 72 hours, respectively. The expression levels of KLK5 mRNA were evaluated by fluorescence quantitative RT-PCR.Results KLX5 mRNA expression levels （KLX5 mRNA/GAPDH mRNA）in MCF-7 and B37 were （3.23 ± 0.51 ） × 10^-5 and （3.13 ± 0.62） × 10^-4, respectively. Compared with ethanol control, KLK5 mRNA expression levels in MCF-7 and B37 were significantly increased with the increase of 17-βE2 concentration （ P 〈 0.01 ）, while showed no statistical change when 17-βE2 concentration was below 10^-11 mol/L（ P 〈 0.05）. Conclusion Estrogen up-regulated the expression levels of KLX5 mRNA in MCF-7 and B37 in a dose dependent way.
作者 陈英剑 胡成进 闻新棉 徐军 孙晓明
机构地区 济南军区总医院实验诊断科
出处 《中国实验诊断学》 2007年第8期1005-1008,共4页 Chinese Journal of Laboratory Diagnosis
关键词 乳腺癌 组织激肽释放酶5基因(KLK5) 雌激素 breast cancer kallikrein 5（KLK5） estrogen
分类号 R737.9 [医药卫生—肿瘤]
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参考文献10

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共引文献15

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中国实验诊断学
2007年 第8期

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