葡萄糖苯氮芥衍生物的合成及抗肿瘤活性的研究

Study of synthesis and anti-tumor activities of the benzene nitrogen mustard glucoside derivate

目的探讨新合成的葡萄糖苯氮芥衍生物对肝癌7721细胞的生长抑制及其诱导凋亡的作用。方法采用MTT,流式细胞术分析法。结果新合成的葡萄糖苯氮芥衍生物对正常细胞毒性较小,对7721有明显的细胞毒性,且通过流式细胞仪显示其诱导细胞凋亡。结论新合成的葡萄糖苯氮芥衍生物能明显抑制肝癌细胞的生长,诱导细胞凋亡是其抗肿瘤作用的主要机制之一。

Objective To analyze the new synthesis benzene nitrogen mustard glucoside derivate inhibit the growth and induce the apoptosis of 7721 hepatumor cells. Methods The experiments were used by MTr and FCM. Results The new synthesis benzene nitrogen mustard glucoside derivate is much lower toxicity to normal cellsd and much higher toxicity to 7721 cells ＇compared with nitrogen mustard, meanwhile it induce the apoptosis of 7721 cells by the FCM. Conclusion The new synthesis benzene nitrogen mustard glucoside derivate can inhibit the growth of 7721 hepatumor ceils and induce the apoptosis which is the one of the main mechanisms of antiturnour.