结直肠癌患者外周血CEA-mRNA表达的研究

Study on the expression of carcinoembryonic antigen mRNA in the peripheral blood of patients with colorectal cancer

目的检测结直肠癌患者外周血中的循环肿瘤细胞癌胚抗原(CEA)mRNA的表达,并分析其临床价值。方法40例有明确临床肿瘤病灶存在的结肠癌患者,20例结肠息肉患者和40例健康志愿者,分别以逆转录PCR(RT-PCR)检测其外周血中CEA mRNA的表达。结果40例结肠癌患者外周血中CEA mRNA表达的阳性率为45.0%(18/40),而健康对照组均为阴性,两者比较有显著性差异(P<0.001);20例结肠息肉患者中CEA mRNA表达阳性率为10%(2/20);CEA mRNA阳性表达率与肿瘤分期相关,而与肿瘤细胞分化程度并不相关。结论结直肠癌患者外周血中CEA mRNA的表达的检测对肿瘤的分期、决定治疗手段、判断疗效和预后有着重要的临床价值。

Objective To investigate the carcinoembryonic antigen（CEA） mRNA expression of circulating tumor cells in peripheral blood of patients with colorectal cancer so as to evaluate its clinical significance. Methods 40 patients with colorectal cancer （CRC） ,20 patients with colorectal polyp（CRP）, and 40 healthy volanteers were the subjects. CEA mRNA of circulating tumor cells in peripheral blood was detected by using reverse transcription polymerase chain reaction （RT-PCR）. Results In RT-PCR analysis of the peripherial blood, 45 % （ 1 8 out 40 ） of CRC patients, 10 % （2 out 20） of CRP patients were positive for CEA mRNA, respectively. All 40 healthy individuals were negative for CEA mRNA expression. The detection of CEA mRNA was significantly correlated with TNM stage of CRC patients,while there were no significant relationship between the CEA mRNA expression and the malignant extent of cell differentiation. Conclusion Using RT-PCR for the detection of CEA mRNA is feasible and may be a promising tool for early detection of micrometastic circulating tumor cells in CRC patients,and it possesses clinical significance in assessing the prognosis and scheme of therapy for CRC patients.