乙型肝炎患者特异性CTL细胞数量的研究

Detection of HBcAg-specific cytotoxic lymphocytes in patients with hepatitis B virus

目的检测我国北方地区乙型肝炎患者表位特异性CTL细胞反应水平,研究急性自限性和慢性持续性乙型肝炎患者体内免疫反应的差异。方法使用HBV抗原表位肽五聚体(HBcAg18-27)对急性和慢性乙型肝炎感染患者外周血染色后,用流式细胞仪检测HBV特异性CTL细胞。结果在20例急性乙型肝炎的急性期,可以检测到高水平的HBcAg特异性的CTL细胞,而在急性乙型肝炎的恢复期,HBcAg特异性CTL细胞的水平可以明显下降。在20例慢性乙型肝炎患者中,可以检测到低水平的HBcAg特异性的CTL细胞。结论HBcAg特异性CTL细胞在急性肝炎患者清除乙型肝炎病毒的过程中可能起到至关重要的作用。

Objective To investigate the difference of host immune response specific to hepatitis B virus（HBV）infections between acute self limited and chronic persistent hepatitis in the northern part of the country by quantitative analysis of HLA-A ＊ 0201- rcstricted HBeAg specific cytotoxic T lymphocyte cells （CTL） .Methods The frequency of HBV specific CTL cells in the peripheral blood from 40 patients infected with HBV were quantified by flow cytometry using one HLA ＊ 0201-HbcAg18-27 peptide pentamer complex. Results High frequencies of circulating HbcAg specific CTL cells were detected in most individuals of 20 patients with acute HBV infection while the number of these cells was significantly reduced at the convalescence stage. Low frequencies of HbeAg specific CTL cells were detected in the peripheral blood from individuals of 20 patients with chronic HBV infection. Conclusion HbeAg-specific CTL cells may play a crucial role in complete clearance of HBV from patients with acute HBV hepatitis.