固相萃取分离富集分光光度法测定水环境中的二价铁

Solid-phase extraction and color measurement for determination of Fe(Ⅱ) in water

提出了应用固相萃取分离富集分光光度法测定水环境中痕量Fe2+的新方法。水样中的Fe2+首先与1,10-邻二氮菲(1,10-phen)络合剂作用生成有色阳离子络合物,然后通过Sep-PakC18柱预富集分离,以乙醇和盐酸混合溶液为洗提液,在509nm测定痕量Fe2+。同时,以人工海水为对象详细讨论了盐含量对分析测定的影响。该方法灵敏度高,选择性好,方法的最低检测限2.9μg/L,相对标准偏差0.93%,可用于测定淡水和海水水样中痕量Fe2+,回收率为92%～102%。

Using solid phase extraction for pre-concentration followed by color measurement, a new analytical method was developed for determination of trace level Fe（Ⅱ ） present in water. Fe（ Ⅱ ） in a water sample was first converted into a colored cationic complex with phenanthlorine; the Fe（Ⅱ ）-phen complex was absorbed on a Sep-Pak C18 cartridge, then extracted by 5 mL mixture of ethanol and HCl, and finally quantified by spectrophotometer at 509 nm. The method＇s detection limit was 2.9 μg/L;the relative standard deviation for 5.0 big Fe（ Ⅱ ） was 0.93%. The new analytical method was applicable to fresh water and sea water;the expected recovery factor was 92%-102%.