糖尿病与正常大鼠视网膜组织显微高光谱图像数据研究

Microscopic Hyperspectral Image Study of Normal and Diabetic Retina Tissues of Rats

使用自行研制的显微高光谱成像仪采集了正常、糖尿病和药物治疗后大鼠的视网膜组织切片的显微高光谱图像数据。通过对正常对照组、糖尿病组、药物治疗3周组和药物治疗6周组4组共60例样本的显微高光谱数据进行处理,提取其外核丛透射光谱曲线进行对比分析发现,大鼠视网膜外核丛组织在400-800 nm光谱范围内存在3个谱峰,且正常大鼠视网膜外核丛组织光谱曲线在第113波段的谱峰明显高于糖尿病大鼠相同位置的谱峰。另外,经过注射LCVS1001药物治疗后大鼠视网膜外核丛组织的光谱曲线有逐渐向正常光谱曲线转变的趋势。通过该研究表明,可以利用显微高光谱成像仪来研究糖尿病大鼠视网膜的光谱特征以及物理化学组成等变化。

A microscopic hyperspectral imaging device was used to study the normal and diabetic retinas of rats. The microscopic hyperspectral images of retinas from normal, diabetic and medicative rats were collected and processed. Some transmission spec- tral curves of outer nuclear layer of retina were extracted and analyzed. The results showed that there are three characteristic spikes in the spectral curves between 400 and 800 ran from the outer nuclear layer, and the heights of the characteristic spikes at 113 band were altered obviously between the normal and diabetic retinas. In addition, the spectral curve of the diabetic retina changes gradually to the normal spectrum curve after treatment by the LCVS1001. The experiment result showed that the microscope hyperspectral imaging device could be used to study the changes in spectrum characters and physical chemistry composition of the diabetic retina.