流动注射化学发光法测定芦丁

Determination of Rutin by Flow Injection Chemiluminescence Method

在酸性介质中,硫酸高铈氧化芦丁能产生弱发光,而罗丹明6G（Rh6G）能大大增强此弱发光,由此建立了流动注射化学发光法测定芦丁的新方法。研究了影响化学发光强度的各种因素,优化了硫酸、硫酸高铈和Rh6G等条件,对样品中可能存在的各种干扰物质进行了研究,并初步探讨了化学发光反应可能的机理。该方法的线性范围为1.0×10^-7-1.0×10^-4mol·L^-1,方法的检出限为8.1×10^-8mol·L^-1,对1.0×10-5mol.L-1的芦丁进行11次平行测定,其相对标准偏差为1.5%,回收率为99.0%-104.0%。该方法应用于芦丁片剂中芦丁含量的测定,取得了满意的结果。

A new flow injection chemiluminescence method for the determination of rutin was established. The method was based on the chemiluminescence reaction of rutin with cerium （IV） sulfate in sulfuric acid condition sensitized by rhodamine 6G. Various factors affecting the chemiluminescence intensity of the system were investigated, and the condition of sulfuric acid, cerium （IV） sulfate and Rhodamine 6G etc was optimized. We have done research on all kinds of possible disturbers in the sample. The possible mechanism of the proposed method was also studied. The method is simple, convenient and sensitive. The linear relationship between the intensity of chemiluminescence and the concentration of rutin is over the range of 1.0×10^-7-1.0×10^-4mol·L^-1. The detection limit is 8.1×10^-8mol·L^-1, the relative standard deviation is 1.50/00 for 11 measurements of 1.0×10^-5ol·L^-1andard solution. This method has been successfully applied to the determination of rutin in tablets with recoveries between 99.0% and 104.0%.