芸香对人膀胱癌EJ细胞体外作用的研究

Effects of quercetin on human bladder cancer cell line EJ

目的:探讨芸香对人膀胱癌EJ细胞的作用。方法:MTT法检测EJ细胞生长抑制率;流式细胞仪检测细胞周期改变和免疫组化SP法检测Ki-67蛋白表达的变化。结果:芸香浓度为30、60、90μmol/ml时,EJ细胞的生长受到不同程度的抑制,且具有时间和浓度依赖性。EJ细胞经不同浓度的芸香作用24h后G0/G1期细胞比率明显下降,而G2/M期比率明显上升。EJ细胞Ki-67的阳性率随芸香浓度的增高而下降(P<0.05)。结论:芸香能抑制癌细胞的生长增殖。其机制可能通过诱导癌细胞周期阻滞于G2/M期、下调Ki-67表达有关。

To study antitumor mechanisms of quercetin on human bladder cancer cell line EJ in vitro. Methods：Cell suppression rates were treated by MTT assay, cell cycle was measured by flow cytometry and Ki-67 expression was detected by immunohistochemistry. Results：Different levels of cell suppression rates were observed while EJ cell were treated with different concentrations（30.60.90/mol/ml） of quercetin, and in a time and concentration dependent manner. The ratio of Go/Gl phase cells was decreased and the ratio of G2/M phase cells was increased, when treated with 30.60.90 μmol/ml quercetin for 24 h. The expression of Ki-67 was down-regulated when treated with 30.601.90 μmol/ml quercetin. Conclusions： Quercetin could inhibit human bladder cancer EJ cell growth. The possible mechanism was to induce cell cycle arrest at G2/M phase and down-regulate the expression of Ki-67 protein.