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次级淋巴组织趋化因子浓度梯度抑制肿瘤细胞免疫逃逸 被引量：2

Effects of SLC concentration gradient on suppression of tumor cell immune escape
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摘要 目的探讨人次级淋巴组织趋化因子(SLC)浓度梯度对肿瘤细胞免疫逃逸的影响。方法根据SLC浓度梯度,实验分6组;流式细胞术检测肿瘤细胞HLA-Ⅰ类分子的表达、肿瘤细胞凋亡,Western blot检测肿瘤细胞胞内BCL-2表达,ELISA检测肿瘤细胞培养上清中生长转化因子β(TGF-β)水平。结果在一定浓度范围内,随SLC浓度增加,肿瘤细胞的HLA-Ⅰ类分子表达增高,肿瘤细胞凋亡增多;BCL-2表达增高,而肿瘤细胞TGF-β分泌水平降低。结论SLC具有抑制肿瘤细胞免疫逃逸的作用。 Objective To explore the effects of SLC concentration gradient on suppression of tumor immune escape. Methods According to different SLC concentration, there were six groups. The HLA- Ⅰ expression and apoptosis of MCF-7 were detected with FCM, and＇intracellular BCL-2 expression was analyzed by western blot. The production of TGF-β was detected with ELISA. Results In a certain range of concentration gradient, following SLC increase, HLA- Ⅰ expression level on MCF-7 was improved, and apoptosis was induced but BCL-2 expression was enhanced. Moreover, the secretion of TGF-β was suppressed. Conclusion SLC inhibites tumor immune escape.
作者 吴砂 袁晓梅 朱慧芬 沈关心
机构地区 华中科技大学同济医学院免疫学系
出处 《基础医学与临床》 CSCD 北大核心 2007年第8期846-848,共3页 Basic and Clinical Medicine
基金 国家高新技术研究发展计划(2006AA02Z158) 教育部博士点专项资金(20060487024)
关键词 次级淋巴组织趋化因子 肿瘤细胞 免疫逃逸 secondary lymphoid tissue chemokine tumor cell immune escape
分类号 R730.3 [医药卫生—肿瘤]
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参考文献11

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二级参考文献24

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基础医学与临床
2007年 第8期

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