摘要
目的观察脑室注射孤啡肽和吗啡对大鼠皮层体感诱发电位(SEP)和Na+-K+ATP酶活性的影响,探讨孤啡肽在脑内致痛觉过敏和抗吗啡镇痛作用的可能机制。方法健康Wistar大鼠,用生物信号采集系统经硬膜外引导SEP;用ATP酶测试盒,检测大鼠皮层体感区组织匀浆上清液中Na+-K+ATP酶的活性。结果1)脑室注射0·9μg的孤啡肽后,皮层SEP的P1-N1峰峰值和N1-P2峰峰值波幅明显增加(P<0·01)。脑室分别注射0·04μg、0·45μg、0·9μg、1·8μg的孤啡肽,可剂量依赖性的抑制Na+-K+ATP酶的活性(P<0·01)。2)脑室注射20μg吗啡后,皮层SEP明显被抑制,Na+-K+ATP酶活性显著增加(P<0·01)。3)脑室联合注射孤啡肽(0·9μg)和吗啡(20μg)后,皮层SEP和Na+-K+ATP酶活性均无明显变化。结论孤啡肽在中枢可能通过影响SEP和Na+-K+ATP酶产生痛觉过敏和抗吗啡镇痛作用。
Objective To investigate the effect of intracerebroventricular injection of morphine and orphanin FQ （OFQ） on somatosensory evoked potential （SEP） and Na^＋-K^＋ ATPase activity in cerebral cortex of rat. Methods To study the SEP with the BL-420 biological signal collecting system. The cerebral cortex tissues were extracted, homogenized and centrifuged. Na^＋-K^＋ ATPase activity was measured with ATPase analytical kit. Results After intracerebroventricular injection with OFQ 0. 9 μg, the amplitude of P1-N1 and N1-P2 increased significantly （P 〈 0. 01 ）. After intracerebroventricular injection with OFQ （0. 04, 0. 45, 0. 9, 1.8μg）, the Na^＋-K^＋ ATPase activities were significantly decreased, with the increase of concentration of OFQ （P 〈0. 01 ）. After intracerebrov-entricular injection with morphine （20 μg）, the amplitude of P1-N1 and N1-P2 significantly decreased and the activity of Na^＋-K^＋ ATPase significantly increased （P〈0.0I）. After intracerebroventricular injection with OFQ （0. 9 μg） and morphine （20 μg）, the amplitude of SEP and the Na Na^＋-K^＋ ATPase activity were not significantly different as compared with control groups. Conclusion The effect of OFQ is related to the changes of the SEP amplitude and Na Na^＋-K^＋ ATPase activity in CNS.
出处
《基础医学与临床》
CSCD
北大核心
2007年第8期868-871,共4页
Basic and Clinical Medicine
基金
黑龙江省卫生厅资助项目(2003-097)
