卵巢癌组织及细胞株中肿瘤特异性抗原MAGE、GAGE、BAGE基因表达的研究

Expression of tumor specific antigen MAGE,BAGE,GAGE in ovarian cancer tissues and cell lines

目的:观察卵巢上皮性癌组织及细胞株中肿瘤特异性抗原MAGE、BAGE、GAGE基因的表达,以探讨基因表达与诊断、治疗及预后的关系,评价基因产物作为卵巢癌标志物以及卵巢癌免疫治疗靶位的可行性。方法:采用RT-PCR分析10例正常卵巢2、0例卵巢良性肿瘤、47例卵巢上皮性癌组织及3种卵巢癌细胞株SKOV3、A2780、COC1中MAGE-1、MAGE-3、GAGE-1/2及BAGE基因mRNA水平的表达。结果:正常卵巢组织中MAGE、GAGE、BAGE基因无表达,良性肿瘤中仅有MAGE-1基因表达,阳性率为15%(3/20)。卵巢癌组织MAGE-1、MAGE-3的表达率较高,分别为51.1%(25/47)、34%(16/47),GAGE-1/2及BAGE基因在卵巢癌组织中的表达率分别为25.5%(12/47)和14.9%(7/47)。卵巢癌转移灶仅有MAGE-1、BAGE基因表达,阳性表达率分别为28.6%(2/7)、14.3%(1/7)。浆液性囊腺癌MAGE-1、MAGE-3基因阳性表达率明显高于其它类型卵巢恶性肿瘤(P<0.05)。结论:肿瘤特异性抗原MAGE、GAGE、BAGE基因在卵巢癌中有较高表达,其表达与有无淋巴结转移无关,MAGE-1、MAGE-3基因的阳性表达与肿瘤分化程度及临床分期密切相关,伴有腹水的卵巢癌患者BAGE表达的阳性率较高。卵巢癌细胞株SKOV3、A2780、COC1的表达谱不尽相同,但至少有一种基因表达,其可作为卵巢癌免疫治疗的特异性靶点。

Objective： To explore the expression of MAGE, BAGE and GAGE genes in ovarian cancer tissues and cell lines. Methods： Expression levels of MAGE-1, MAGE-3, GAGE-1/2 and BAGE were determined by reverse transcription polymerase chain reaction（FF-PCR） in ovarian cancer cell lines SKOV3, A2780, COC1, and 10 cases of normal ovarian tissues, 20 cases of benign epithelial tumor, and 47 cases of malignant epithelial tumor. Results： Normal ovarian tissues did not express members of the above gene families. In benign tumor tissue specimens, only 3 cases expressed MAGE-1 （3/20）. MAGE-1, MAGE-3, GAGE-1/2 and BAGE was expressed in 25（51.1%）,16（34%）, 12（25.5% ）, 7（14.9%） cases of 47 ovarian cancer tissues. Conclusion： High expression of MAGE, BAGE and GAGE genes is produced in ovarian cancer tissues, which is related to the tumor grade, clinical stage, ascites, and the histological subtype, but is not related to age and lymph node metastasis. There was at least one of these genes expressed in ovarian cancer cell line SKOV3, A2780, and COC1. These tumor-specific antigens can be used as molecular markers for diagnosis and possible targets for immunotherapy.