促红细胞生成素对血管性痴呆大鼠胆碱能神经纤维保护作用的研究

Protective effect of erythropoietin on the expression of cholinergic neural fiber in the vascular dementia rat model

目的:研究促红细胞生成素(Epo)对血管性痴呆(VD)大鼠胆碱能神经纤维的保护作用,探讨EPO对脑缺血后损伤的保护作用及其机制。方法:将大鼠随机分为假手术组、用药组和模型组,每组12只。用药组和模型组按"两血管阻断+硝普钠降压"法建立VD大鼠模型,并给予Epo治疗,采用Morris水迷宫实验、Nissl染色、乙酰胆碱酯酶(AchE)组化染色和AchE活性测定法检测Epo对学习记忆、细胞形态和AchE表达和活性的作用。结果:与假手术组和用药组相比,Epo明显缩短VD模型组大鼠Morris水迷宫中的逃避潜伏期,显著增加了海马CA1区锥体细胞及尼氏小体数量,并且显著提高了AchE免疫阳性细胞数量和AchE活性(P<0.01,P<0.05)。结论:EPO可改善VD大鼠行为学表现和病理损害,其机制可能与保护胆碱能神经纤维有关。

Objective： To study the protective effect of erythropoietin（Epo） on the expression of cholinergic neural fider in vascular dementia（VD） mt model. Methods： VD rat models were established by two-vessel occlusion and salium nitropnmside and they were treated by EPO. The Morris labyrinth, Nissl, acetylcholinesterase（AchE） histochemistric staining and AchE activity assessment methods were used to deteimine the influence of EPO on the study and memory, cellular morphous and AchE expression. Results： Compared with the sham-operative group and the Epo-treatment group, EPO significantly shortened the escaping latency of the Morris labyrinth and increased the number of neurons, Nissl bedies, AchE positive neurons fibers and AchE activity in the hippocampus and frontal cortex of the VD model group（ P 〈 0.01 and P 〈0.05）. Conclusion： Epo can improve the insults of praxiology and pathology of VD rats, which may be related to its protection of AchE neural fibers.