复方水蛭素的药效学研究

Pharmacodynamics of compound hirudin

目的:探讨复方水蛭素抗肿瘤的作用机理。方法:①对W256肿瘤的影响:于大白鼠左前腋下接种W256瘤种细胞液(0.3 ml/只),次日开始用药。阴性对照组给予生理盐水;阳性对照组给予5-FU;中药组分别给予不同剂量的复方水蛭素;②对胃癌靶细胞(NKM)的影响:将药物稀释成1∶10、1∶20、1∶40、1∶80、1∶160、1∶320、1∶640的浓度,接种于多孔培养板内的人胚胎肺细胞(HEL)和肿瘤靶细胞(NKM)上,37℃、CO2培养,观察细胞病变,计算抑瘤指数;③对超氧化物歧化酶(SOD)和脂质过氧化物(MDA)的影响:取小鼠44只,随机分成四组,给药方法同W256肿瘤实验组,连续用药30 d,次日摘取小鼠眼球取血,用微量快速检测法测定SOD和MDA的含量。结果:复方水蛭素对W256肿瘤具有明显的抑制作用(P<0.01),该药浓度在1∶80(最大抑瘤浓度)时抑制肿瘤靶细胞的效应最大,1∶640(最小抑瘤浓度)时,抑制肿瘤靶细胞的效应最小。同时,复方水蛭素还能明显提高小鼠血中SOD的活性和降低MDA代谢产物的含量(P<0.05)。结论:复方水蛭素具有明显的体内外抗肿瘤作用。

Objective： To investigate the anti-tumor mechanism of hirudin. Methods： （1）W256 tumors were transplanted into the left armpit of rats. Then they were divided into three groups： the negative control group was subjected to saline, the positive control group was subjected to 5-Fu, and the traditional Chinese medicine group was subjected to compound hirudin. （2） The drug was diluted to a ratio of 1 ： 10, 1：20,1 ：40, 1：80, 1 ： 160, 1：320, and 1：640 and was implanted into a porous plate for HEL and NKM under a 37 ℃ and CO2 condition. The inhibition effect was determined. （3） 40 mice were also divided inlo groups as the first experiment. 30 days later, the SOD and MDA contents were deter- mined in the eyeball blood by a trace rapid assay. Results： Compound hirudin effectively inhibited the growth of W256 tumors（P 〈0.01）and was the most significant at a ratio of 1：80 and was the least significant at a ratio of 1：640. It also can significantly increase SOD activity and reduce MDA metabolity（ P 〈 0.05）. Conclusion： Compound hirudin can significantly affect the W256 tumor in vivo, the NKM in vitro and the blood SOD and MDA.