罗格列酮抑制垂体腺瘤GH3细胞及促凋亡作用的研究

Rosiglitazone induces growth inhibition and apoptosis of pituitary GH3 cells

目的探讨罗格列酮对GH3细胞增殖的影响及其促细胞凋亡的机制。方法不同浓度罗格列酮作用GH3细胞后,流式细胞仪检测细胞周期和细胞凋亡,Elisa法分析罗格列酮对生长激素合成的影响,Western blot法分析GH3细胞Cyclin D3、bcl-2和bax的变化。结果罗格列酮作用GH3细胞48 h后,以浓度效应关系抑制GH3细胞增殖、并使细胞增殖周期中G0-G1期阻滞,S期和G2-M期百分率降低;Western blot法示罗格列酮作用GH3细胞提高了Cyclin D3和bax的表达,而bcl-2的表达降低。结论罗格列酮促GH3细胞凋亡并抑制GH分泌,可能成为垂体生长激素腺瘤病人新的治疗方法。

Objective To explore the effects of rosiglitazone on rat GH-secreting （GH3） cell proliferation and its role in apoptosis. Methods After different concentrations of rosiglitazone acted on GH3 cells, the cell cycle and apoptosis were assessed by FACS analysis. The effects of rosiglitazone on GH synthesis were evaluated by ELISA. Expressions of cyclin D3, bcl-2 and bax were evaluated by Western blotting. Results After treatment of CH3 cells with rosiglitazone for 48 h, the cell proliferation was inhibited in a concentration-dependent manner. G0-G1 cell cycle phase was blocked and cell proportion in the S and G：-M phase decreased significantly. Rosiglitazone increased the expression of cyclin D3 and bax, decreased the expression of bcl-2. Conclusion Rosiglitazone suppress GH secretion as well as apoptosis and has the potential of being used to treat pituitary growth hormone-secreting adenomas