摘要
以二倍体、四倍体和六倍体冰草(P基因组)以及中国春、Fukuho、栽培一粒小麦和硬粒小麦(ABD基因组)等为材料进行RAPD分析,从520个RAPD随机引物中,筛选出2个P基因组特异的RAPD分子标记OPC04和OPP12.利用OPC04和OPP12对小麦族其它基因组植物和8个小麦-冰草二体附加系进行扩增,结果表明OPC04和OPP12在ABD、C、E、AG、I、M、R、S、V、Y等基因组扩增中未出现相应结合位点;而在对8个小麦-冰草二体附加系扩增中均出现此2个特异片段,且扩增稳定、重复性好.进一步表明OPC04和OPP12是冰草P基因组的特异标记,可作为小麦-冰草重组系外源P染色质的鉴定标记之一.
A total of 520 decamer oligonucleotides were used to perform random amplified polymorphic DNA （RAPD） analysis on Z804,Z559 ,Z1750,Chinese Spring,Fukuho,T. monococcum and T. durum. Two P genome specific markers, OPC04 and OPP12 were screened. These two specific primers were then used to amplify on other genome of Triticeae, and 8 wheat-Agropyron cristatum disomic addition lines. The results showed that there is no PCR amplification on ABD, C, E, AG, I, M, R, S, V, Y genome by primer OPC04 and OPP12. However,there are PCR products in all 8 wheat-Agropyron cristatum disomic addition lines. The results further indicated that OPC04 and OPP12 were the specific amplifications for the P genomes. Therefore,OPC04 and OPP12 could be the specific markers on detecting the chromatin of P genome in the wheat-Agropyron progeny.
出处
《西北植物学报》
CAS
CSCD
北大核心
2007年第8期1550-1557,共8页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金项目(30470968)
关键词
冰草
P基因组
RAPD
特异标记
Agropyron cristatum
P genome
RAPD
specific markers
