外源H_2O_2胁迫对大蒜试管苗玻璃化的影响

Vitrification of the Garlic Plantlet under Exogenous H_2O_2 Stress in vitro

以大蒜品种二水早为材料,研究不同浓度外源H2O2胁迫对大蒜试管苗的玻璃化发生及生理生化变化的影响.结果表明,在不同浓度外源H2O2处理下,大蒜玻璃化试管苗百分率、组织含水量、MDA含量、电解质渗透率、SOD和POD活性均高于对照,且随H2O2浓度的增加而升高,叶绿素含量则表现相反的趋势;在同一H2O2浓度下,大蒜玻璃化试管苗的组织含水量、MDA含量、电解质渗透率、SOD、POD和CAT活性均显著高于大蒜正常试管苗,叶绿素含量低于正常试管苗.研究发现,外源H2O2胁迫对大蒜试管苗玻璃化有促进作用.

Using the garlic variety＇Ershuizao＇as test material,the effects of different concentrations of exogenous H2O2 stress on occurrence of vitrification and the physiological and biochemical change in the garlic plantlet in vitro were studied in this paper. The results showed that, under different concentrations of exogenous H2O2 ,the vitrification plantlet rates, tissue water content, MDA content, rate of electrolyte leakage, SOD activity and POD activity were higher than that of control. But the chlorophyll content decreased with the increase of the concentration of exogenous H2O2. At a certain exogenous H2O2 concentration,the vitrification plantlet rates, tissue water content, MDA content, rate of electrolyte leakage, SOD activity, POD activity and CAT activity of vitreous plantlet were significantly higher than that of the normal plantlet,while the chlorophyll content of vitreous plantlet was lower than that of the normal one. The results revealed that exogenous H2O2 stress can enhance the effectively vitrification of the garlic plantlet in vitro.