温和灸对中老年部分雄激素缺乏综合征大鼠模型影响的实验研究

Influence of Mild Moxibustion on Androgenic Hormone in Male Rats with Partial Androgen Deficiency

目的:观察温和灸对于中老年部分雄激素缺乏综合症(PADAM)模型大鼠生殖内分泌的影响。方法:根据测定大鼠血清的总睾酮(TT)和血清游离睾酮(FT)的水平,选择符合标准PADAM的中老年SD大鼠模型30例,按照随机分组的原则分为3组:艾灸组(灸"肾俞""命门""关元")、对照组Ⅰ(丙酸睾丸素组)、对照组Ⅱ(模型组),并测定各组治疗后血清中TT和FT的水平,观察各组大鼠一般情况的变化及通过悬尾实验和力竭游泳实验及取得其相关脏器指数等评价大鼠的自身状况,进行统计分析。结果:两治疗组经治疗后血清中TT、FT的含量较治疗前均显著提高(P<0.01),两组之间无显著性差异(P>0.05),而模型组FT显著下降(P<0.05)。两治疗组经治疗后悬尾实验不动时间均显著缩短(P<0.05),力竭游泳实验时间与模型组比较显著延长(P<0.01)。结论:温和灸法可阻止PADAM大鼠血TT、FT的下降,减轻腹部脂肪堆积,调节免疫功能,改善抑郁状态和肌张力,其作用与丙酸睾丸素相当。

Objective ： To observe the effect of mild moxibustion on the reproductive endocrine in rats with partial androgen deficiency （PAD）. Methods： Thirty male SD rats （aged 12 months） were randomized into control, androlin （testosterone propionate） and moxibustion E＂Shenshu＂ （BL 23）, ＂Mingmen＂ （GV 4） and ＂Guanyuan＂ （CV 4）1 groups. PAD was determined by comparing these aging rats （n = 50） with young rats （n = 30） in serum total testosterone （TT） and free testosterone （FT） contents which were detected with radioimmunoassay. At the end of experiments, the rats＇ spleen, testis, thymus, and perirenal fat weight were detected respectively for calculating visceral index （each viscera weight/body weight）. The rats＇ tail suspension tests were conducted for calculating the animal immobility duration in 6 min, and exhaustion swimming tests carried out for analyzing the duration of exhaustion swimming （from placing them into a water pool to the time when the animal sunk into water for 6 s）. Results： 1） After the treatment, serum TT and FT contents in moxibustion and androlin groups increased significantly compared with pretherapy in each group （P〈0.01） and compared with control group （P〈0.05, 0.01）. 2） Results of rats＇ tail suspension tests displayed that after the therapy, the duration of immobility in moxibustion and androlin groups decreased markedly in comparison with pro-treatment of each group （P〈0.05） and with control group （androlin group, P〈0.05）. 3） Compared with control group, the duration of exhaustion swimming of moxibustion and androlin groups increased considerably （P〈0.01）. No significant differences were found between moxibustion and androlin groups in serum TT and FT levels, immobility duration and the duration of exhaustion swimming （P〉0.05）. 4） Compared with control group, the indexes of thymus and testis raised remarkably, and that of perirenal fat lowered evidently （P〈0. 05, 0.01 ）, while no significant difference was found between moxibustion and androlin groups in indexes of thymus, testis and perirenal fat （P〉0.05）. There existed significant correlation between visceral index （thymus, testis and perirenal fat） and serum TT and FT levels respectively （P〈0. 01 ）, and between the duration of immobility and exhaustion swimming and serum TT and FT levels separately （P〈0.01）. Conclusion： Mild moxibustion may prevent blood TT and FT levels from decline, reduce fat accumulation in the abdomen, regulate immune function, improve the depression state and myodynamia in the aging rats, which is comparable to the effects of testosterone propionate.