山薄荷水提取液对小鼠免疫功能的影响

Influence of Dracocephalum Moldavicum water extract on immunological function in mice

目的:观察山薄荷水提取液对小鼠免疫功能的影响。方法:实验于2005-04/06在赣南医学院药理教研室完成。①取成年昆明种小鼠50只,随机均分成5组,生理盐水组、山薄荷水提取液1,2,4g/kg组、环磷酰胺组。腹腔注射给予相应药物,1次/d,连给7d,末次给药后次日称体质量,麻醉下处死剥取胸腺、脾称质量,计算小鼠的胸腺、脾指数。②取成年昆明种小鼠40只,随机均分成4组,生理盐水组、山薄荷水提取液1,2,4g/kg组。腹腔注射给予相应药物,1次/d,连给10d,于末次给药30min后,尾静脉注射印度墨汁0.01mL/g体质量,注射后1,5min从眶后静脉丛取血20μL,溶于2mL0.1%Na2CO3溶液中,测定吸光度。最后将小鼠麻醉下颈椎脱臼处死,分别称取肝、脾质量,计算廓清指数K和校正廓清指数α。③取成年昆明种小鼠40只,随机均分成4组,生理盐水组、山薄荷水提取液1,2,4g/kg组。每鼠腹腔注射5%生理盐水鸡红细胞混悬液0.2mL进行免疫,即日开始给予相应药物,1次/d,连给7d,摘眼球取血,离心,取血清与鸡红细胞悬液、补体混合,孵育,离心,测定吸光度作为判定血清半数溶血值的指标。④取成年昆明种小鼠50只,随机均分成5组,生理盐水组、山薄荷水提取液1,2,4g/kg组、环磷酰胺组。末次给药后1h,剪鼠尾取血涂片,每只小鼠血涂片标本均查100个淋巴细胞,计算T淋巴细胞的百分比。结果:参加实验的180只小鼠全部进入结果分析,没有脱失。①胸腺、脾指数及T淋巴细胞百分比:山薄荷水提取液2,4g/kg组小鼠胸腺指数、脾指数、外周血T淋巴细胞百分比低于生理盐水组(P<0.05,P<0.01)。而山薄荷水提取液1g/kg组与生理盐水组比较差异无显著性意义(P>0.05)。②校正廓清指数:与山薄荷水提取液2,4g/kg组校正廓清指数低于生理盐水组,差异有显著性意义(P<0.05,P<0.01)。③血清溶血素含量:4g/kg山薄荷水提取液组血清溶血素含量低于生理盐水组,差异有显著性(P<0.01)。结论:中、高剂量山薄荷水提液具有免疫抑制作用。

AIM： To study the effect of Dracocephalum Moldavicum water extract on immunological function in mice. METHODS： The experiment was conducted at the Department of Pharmacology in Gannan Medical College from April to June 2005. ①Fifty adult Kunming mice were randomly divided into five groups, saline group, 1, 2 and 4 g/kg Dracocephalum Moldavicum groups and cyclophosphamide group. The mice were treated with corresponding drugs intraperitoneally, once a day for successively 7 days. After the last administration, body mass was recorded on the following day. The mice were sacrificed to collect and weight thymus gland and spleen, and then mice thymus gland and spleen index were calculated. ②Totally 40 adult Kunming mice were collected and randomly assigned into 4 groups, saline group and 1, 2 and 4 g/kg Dracocephalum Moldavicum groups. The mice were treated with corresponding drugs intraperitoneally, once a day for successively 10 days. Thirty minutes after the last administration, Indian ink （0.01 mL/g） was injected by vena caudalis, 20 μL blood from vena orbitalis posterior was collected at first and fifth minute and dissolved into the solution of 2 mL 0.1% Na2CO3. Absorbance （A） was determined. Then mice were sacrificed by luxating C-spine. The liver and spleen were taken out of and weighed. At last clearance index K and remedying clearance index （x were calculated, respectively. ③Forty adult Kunming mice were randomly divided into 4 groups, saline group and 1, 2 and 4 g/kg Dracocephalum Moldavicum groups. 5% saline and chicken red blood cell （CRBC） suspension （0.2 mL） was injected intraperitoneally and carried out immune in each mouse. Meanwhile, drug was administrated （once a day for 7 days）. Blood was collected by drawing eyeball and then centrifugated. Serum and CRBC suspension and complement were mixed, they were incubated and centrifugated. A was determined as the index of serum hemolysin. ④Fifty adult Kunming mice were randomly divided into five groups, saline group, 1, 2 and 4 g/kg Dracocephalum Moldavicum groups and cyclophosphamide group. One hour after the last administration, blood was collected by cutting tails and made smear. 100 lymphocytes were checked in every blood smear preparation. At last, the percentage of T-lymphocyte was calculated. RESULTS： All 180 mice entered the final analysis without any loss. ①Percentages of thymus gland, spleen index and T-lymphocyte in mice were lower in the 2, 4 g/kg Dracocephalum Moldavicum groups than the saline group （P 〈 0.05, P 〈 0.01 ）. There were no significant differences between saline group and 1 g/kg Dracocephalum Moldavicum group （P〉 0.05）. ②Carbon clearance index was lower in the 2, 4 g/kg Dracocephalum Moldavicum groups than the saline group （P 〈 0.05,P 〈 0.01 ）. ③Serum hemolysin level was lower in the 4 g/kg Dracocephalum Moldavicum group than the saline group （P 〈 0.01 ）. CONCLUSION： Middle and high doses water extract from Dracocephalum Moldavicum has immunosuppressive effect.