不孕不育症患者精子双链DNA的检测

Relationship between Infertility and dsDNA

目的探讨不孕不育症与精子双链DNA的关系。方法采用吖啶橙染色法,染成绿色的是双链DNA。结果对照组120人,其中双链DNA>66%,120例,占100.0%(120/120)。检测组:不孕组840例,其中双链DNA>66%,830例,占98.81%(830/840);双链DNA50±10%;4例,占0.48%(4/840);双链DNA40±10%,3例,占0.36%(3/840);双链DNA30±10%,2例,占0.24%(2/840));双链DNA20±10%,1例,占0.11%(1/840);流产组560例,其中双链DNA>66%,557例,占99.46%(557/560);双链DNA40±10%,1例,占0.18%(1/560);双链DNA30±10%,1例,占0.18%(1/560);双链DNA20±10%,1例,占0.18%(1/560)。对照组(双链DNA)与检测组相比,呈显著性差异(P<0.01)。结论精子双链DNA与不孕(育)症两者相比,呈显著负相关(P<0.01),精子双链DNA降低是引起不孕(育)原因之一。

Objective ：Fo explore the relationship between dsDNA and infertility. Methods Fluorescence detemination by fluorescencent microscope, dsDNA had stained green colour by acridine orange dye. Results dsDNA 〉66% were 100.00% （120/120） in control group（120 cases） . dsDNA 〉66% were 98.81% （830/840）, dsDNA50 ± 10% were 0. 48% （4/840）, dsDNA40 ± 10% were 0. 36% （3/840）, dsDNA30 ± 10% were 0. 24% （2/840）） dsDNA20 ± 10% were 0. 11% （1/840）, in infertility group（840 cases）, dsDNA 〉 66% were 99. 46% （557/560）, dsDNA40 ± 10% were 0. 18% （ 1/560）, dsDNA30 ± 10% were 0. 18% （1/560）. dsDNA20 ±10% were 0. 18% （1/560）, in abortion group （560 case）. There was significant difference between laboratory group （dsDNA） and control group（ P 〈 0.01 ） . Conclusion The dsDNA negatively correlated with fecundity （infertility） （ P 〈0. 01 ）. dsDNA lower can indicate the cause of infertility.