转基因小麦外源优质亚基基因的杂交转育研究

Studies on Transgenes 1Dx5 and 1Ax1 Transferred into Elite Wheat Cultivars by Cross Breeding

在获得外源品质基因1Dx5和1Ax1超量表达的转基因小麦的基础上,利用小麦转基因品系‘B72-8-11b’和‘B102-1-2’为父本,主要以湖北省栽培品种‘鄂麦12’为母本,配置杂交组合。杂交后代中采用系谱选择法,结合HMW-GS鉴定,研究了转基因小麦外源品质基因在F1、F2、F3、F4代的传递,并筛选出外源1Dx5或1Ax1基因保持超表达的2个新型转基因株系;同时证明了将外源品质基因向栽培品种转育,是提高小麦优质亚基含量和提高HMW-GS总量的有效方法之一。

For introducing the transgenes from transgenic wheat lines, two wheat crosses had been made in 2002. In wheat ‘ B72-8-1 lb＇ and ‘ B102-1-2＇ into elite the crosses,elite subunits 1Dx5 and 1Axl were over-expressed in the male parents ‘ B72-8-11 b＇ and ‘ B102-1-2 ＇ respectively, while the female parent ‘ Emai 12 ＇ were selected with better agronomic traits. The expression of exogenous 1Dx5 and 1Axl gene in F1,F2,F3 and F4 generations was tested by SDS-PAGE, then the inheritance patten of the transgenes was analysed. Two lines had been selected from the progenies of the crosses in which the subunit 1 Dx5 or IAxl is over-expressed and the endogenous HMW-GS compositions are different from each other. The results demonstrated that the introduction of transgenes from transgenic wheat lines into elite varieties by crossing is an effective way to transfer extra HMW-GS gene. Key words： Transgenic wheat; Over-expression; New germplasm