流体切应力增加骨髓来源破骨细胞内游离钙离子浓度的研究

Fluid shear stress increases the Ca^(2+) concentration in bone-marrow derived osteoclast-like cells

目的研究流体切应力对培养于载玻片上的破骨细胞内Ca2+浓度的影响。方法应用激光扫描共聚焦显微镜和Fluo-3/AM荧光探针标记技术,测定加力前后破骨细胞内游离Ca2+的荧光强度,获得的图像在计算机上用图像分析软件进行比较分析。结果在37℃、Fluo-3/AM终浓度为10μmol/L的条件下,破骨细胞可获得良好的标记效果,与未加力的对照组相比,流体切应力使破骨细胞内Ca2+荧光信号的平均强度值显著增加。结论体外培养于载玻片上的破骨细胞内Ca2+浓度对流体切应力敏感,Ca2+可介导流体切应力对破骨细胞功能的调节。

Objective To study the change of Ca^2＋ density in cultured osteoclast-like ceils in response to fluid shear stress. Methods Laser scanning confocal microscope and fluorescent probe were used to detect the free Ca^2＋ in osteoclast-like ceils before and after undergoing fluid shear stress. The images were analyzed and compared with image software. Results At 37 ℃ he free Ca^2＋ in osteoclast-like ceils could be labelled effectively with 10 μmol/L Fluo-3/AM. Compared with contol group, the average intensity of Ca^2＋ fluorescent signal in osteoclast-like ceils undergoing fluid shear stress increased significantly. Conclusion The Ca^2＋ concentration in bone-marrow derived osteoclast-like ceils is sensitive to fluid shear stress, which suggests osteoclast-like ceils modulate their function in response to fluid shear stress through the change of free Ca^2＋ concentration.