银杏不同器官蛋白质动态变化的电泳分析

Analysis on dynamicchanges of protein indifferent organs ofGinkgo biloba by SDS-PAGE

利用SDS-PAGE电泳技术对不同季节银杏(Ginkgo bilobaL.)雌雄株叶片和枝条中的贮藏蛋白质组分和可溶性蛋白质含量进行了研究。结果表明,银杏叶片中的可溶性蛋白质含量从10月中旬开始逐渐减少,11月中旬降到最低;枝条中的可溶性蛋白质含量从秋末到整个冬季逐渐增加,进入早春后大幅度下降;同一季节内,叶片中的蛋白质含量明显高于枝条,且雄株叶片和枝条的蛋白质含量高于雌株。单向电泳结果表明,银杏雌雄株叶片中相对分子质量为55 000和32 000的蛋白质谱带均于11月中旬前消失,表明这些蛋白质在银杏落叶前发生了转移。在银杏雌雄株枝条中,相对分子质量为59 000、32 000和27 000的蛋白质谱带均在12月底染色最深,进入早春后消失;雄株枝条中相对分子质量为18 000的蛋白质谱带在12月底前稳定出现,进入早春后消失。表明银杏枝条中的这些蛋白质均表现出冬季积累而春季降解的动态过程。

The protein fraction and soluble protein content in leaf and branch of Ginkgo biloba L. were studied by SDS-PAGE technique. The results showed that soluble protein content in leaf of male and female trees of G. biloba began to decrease at the second ten-days of October, and to the lowest level at the second ten-days of November. While soluble protein content in branch increased gradually from the late of autumn to the late of winter, and then decreased sharply in the early of next spring. In the same season, soluble protein content in leaf was higher than that in branch, and that of male tree was higher than that of female tree. The SDS-PAGE bands of MW （ relative molecular weight） 55 000 and MW 32 000 proteins in leaf could be observed until the second ten-days of November and then disappeared, indicating that these proteins were transformed before leaves falling down. The darkest bands of MW 59 000, MW 32 000 and MW 27 000 proteins in branch were observed before the end of December but disappeared in the early of next spring; MW 18 000 protein band could be observed only male tree and maintained stably before the end of December and disappeared in the early of next spring. It is suggested that these proteins in G. biloba branch would be accumulated in winter and decomposed in spring.