油松卵细胞内蛋白泡动态变化及与卵细胞发育相关特异蛋白质的研究

Protein vacuoles in the egg cells of Pinus tabulaeformis Carr.and specific proteins relating to the development of egg cells

该文采用石蜡切片法对油松卵细胞发育过程中蛋白泡的动态变化进行了研究,并利用蛋白质双向电泳技术和ImageMasterTM2D Platinum软件初步分析了与卵细胞发育相关的特异蛋白.结果表明:5月初颈卵器开始形成,其内充满液泡,随着液泡的消失,蛋白泡从卵细胞的下部和边缘出现,在精核即将进入卵细胞时蛋白泡数量达到高峰,受精时蛋白泡开始减少,逐渐出现空泡现象.精卵结合之后,蛋白泡几乎全部成为空泡.伴随着蛋白泡的变化,胚内的糖类物质和蛋白质也呈现出有规律的时空变化.蛋白质双向电泳图谱表明:从颈卵器形成到卵细胞受精之后,4个新蛋白点出现,10个蛋白点消失,10个蛋白点表达量减弱.

The dynamic variations of protein vacuoles in the egg cells of Pinus tabulaeformis Carr.were studied using the paraffin-cross section method.The specific proteins relating to the development of egg cells were analyzed with two-dimensional electrophoresis（IEF/SDS-PAGE） and ImageMaster^TM 2D Platinum.The results indicated that archegonia,which was filled with vacuoles,emerged at the beginning of May.With the disappearing of vacuoles,many protein vacuoles appeared from the bottom and periphery of egg cells.The number of protein vacuoles reached high peak just before fertilization,and the number and volume of protein vacuoles decreased when the egg cells were fertilized.After sperm-oocyte fusion,protein vacuoles disappeared immediately.At the same time,saccharides and proteins changed regularly in the embryos.Furthermore,there were four new proteins appeared,ten proteins disappeared and the other ten proteins decreased during the development from archegonia formation to fertilized egg cells.